Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream,Acanthopagrus schlegeli

作     者：JI Hong OM Ahamd Daud YOSHIMATSU Takao UMINO Testuya NAKAGAWA Heisuke FURUHASHI Makoto SAKAMOTO Shuichi 

作者机构：College of Animal Science and Technology Northwest Agriculture and Forestry University Shaanxi 712100 China Graduate School of Biosphere Science Hiroshima University Higashi - hiroshima 739 - 8528 Japan Marine Finfish Production and Research Center Terengganu 22200 Malaysia Farming System Division National Research Institute of Aquaculture Fisheries Research Agency Mie 516 -0913 Japan Oriental Yeast Co Ltd Chiba 281 -0002 Japan 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2007年第26卷第1期

页      面：112-121页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：日本文部科学省项目 

主　　题：DHA enzyme lipogenesis lipolysis black sea bream 

摘      要：Hatchery-roared juvenile black sea breams are characterized by a low level of highly unsaturated fatty acids in their bodies, as compared with wild fish. To assess the effect of docosahaxaenoic acid （DHA） on lipegenic and lipelysis enzymes, one-year fish were roared on a casein-based purified diet and a DHA fortified diet （ 1.5% DHA ethyl ester/kg diet） for 60 d, followed with a period of 55 d for starvation. Dietary DHA was effectively incorporated into the fish body. Fortification of DHA depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancroas and intraperitoneal fat body. Carnitine palmitoyltransferase as lipolysis enzyme in the hepatopancreas was active in the DHA fortified fish. Starvation after feeding experiment induced increased carnitine palmitoyltransferase activity in both control and DHA fortified fish and the activity remained higher in the DHA fortified fish, while the monoenes were selectively consumed prior to highly unsaturated fatty acids. These results indicated that dietary DHA depressed lipogenesis and activated lipolysis.