Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta

作     者：Garett Dunsmore Petya Koleva Reed Taylor Sutton Lindsy Ambrosio Vivian Huang Shokrollah Elahi 

作者机构：Department of Dentistry and Department of Medical Microbiology and ImmunologyUniversity of Alberta Department of DentistryUniversity of Alberta Division of GastroenterologyUniversity of Alberta Division of GastroenterologyUniversity of TorontoMount Sinai HospitalSinai Health System 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2018年第24卷第42期

页      面：4787-4797页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：a Foundation Scheme grant and a New Investigator Salary Award from the Canadian Institutes of Health Research(both to Elahi S) an innovation grant from Women and Children’s Health Research Institute(to Huang V and Elahi S) 

主　　题：Caesarian section Twins Vaginal delivery Immunophenotyping CD71+erythroid cells Cord blood Placental tissues 

摘      要：AIM To understand the effects of delivery mode on the immune cells frequency and function in cord blood and *** We evaluated immunological differences in cord blood and placental tissues for a case of twins one of which delivered vaginally while the other delivered by caesarian section(C-section). Cord blood mononuclear cells were isolated and placenta tissues were processed for cell isolation. Immune phenotyping was performed by flow cytometry methods following staining for T cells, natural killer(NK) cells, monocytes, neutrophils and CD71^+erythroid cells in both cord blood and placenta tissues. In addition, fetal calprotectin of twins was measured 12 wk after *** We found lower percentages of immune cells(e.g. T cells, monocytes and neutrophils) in the cord blood of C-section delivered compared to vaginally delivered newborn. In contrast, percentages of monocytes and neutrophils were 2 folds higher in the placental tissues of C-section delivered newborn. More importantly, we observed lower percentages of CD71^+ erythroid cells in both cord blood and placental tissues of C-section delivered case. Lower CD71^+ erythroid cells were associated with a more pro-inflammatory milieu at the fetomaternal interface reflected by higher expression of inhibitory receptors on CD4^+ T cells, higher frequency of monocytes and neutrophils. Furthermore, type of delivery impacted the gene expression profile in CD71^+erythroid cells. Finally, we found that C-section delivered child had 20-fold higher FCP in his fecal sample at 12 wk of *** Mode of delivery impacted immune cells profile in cord blood/placenta. In particular frequency of immunosuppressive CD71^+ erythroid cells was reduced in C-section delivered newborn.