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immunophenotyping of Lymphocyte T and B in the Peripheral Blood of Systemic Lupus Erythematosus

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第2期21卷 108-109页

作者：胡绍先陶德定何培根 Department of Rheumatology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Molecular Medicine Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan

The immunophenotyping expression levels of lymphocyte in the peripheral blood from 21 patients with active systemic lupus erythematosus were analyzed by using the immunofluorescence labeling flow cytometry technique to investigate the immunophenotyping expression of lymphocytes T and B in the peripheral blood of active SLE patients and its clinical value. It was showed that, compared with normal controls, the expression of CD + 3, CD + 4 and the ratio of CD + 4/CD + 8 in the peripheral blood of these patients were decreased , while the expression of CD + 8, CD + 20 was significantly increased . It was suggested that both T and B cells in patients with active SLE involved in immunoregulation, were activated. The abnormal expression of lymphocyte immunophenotyping could influence the immune reaction in SLE patients, which might be one of the important pathogenesis factors in SLE.

关键词： systemic lupus erythematosus immunophenotyping

Principle of panel design,antibody selection,and fluorochrome choice for immunophenotyping of acute leukemia

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国际检验医学杂志 2011年第15期32卷 1698-1700页

作者： Betty Li Clinical Flowcytometry Lab Brigham & Women′s Hospital Boston

After working in the field of clinical flow cytometry for many years,I still continue wondering at its ever changing and evolving nature.Like everything else,clinical flow cytometry comes a long way,and I am sure will... 详细信息

关键词： immunophenotyping acute leukemia panel setup

immunophenotypings of Malignant Epithelial Mesothelioma and Their Roles in the Differential Diagnosis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第2期24卷 112-115页

作者：阮秋蓉胡余昌 Department of Pathology School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology

To investigate the immunophenotypings of malignant epithelial mesothelioma (MEM), and to seek the valuable markers in distinguishing peritoneal MEM from peritoneal metastatic ovarian adenocarcinoma (OA) and colorectal adenocarcinoma (CA), immunohistochemical SP method was used to detect expressions of HBME-1, E-cadherin, CA19-9, MOC-31 and CK7 in paraffin-embedded tissues of 18 cases of MEM, 20 OA and 20 CA. The results showed that there was a significant difference in the expressions of E-cadherin, CA19-9 and MOC-31 between MEM and OA group (P<0.05). Similarly, the difference in the expression of HBME-1, E-cadherin, CA19-9, MOC-31 and CK7 between MEM and CA groups is significant (P<0.05). These results indicate that HBME-1 could be used as a positive marker in distinguishing MEM from CA. E-cadherin, CA19-9 and MOC-31 are considered to be useful negative markers in diagnostic distinction between MEM and metastatic adenocarcinomas, including OA and CA. CK7 is the best positive marker in distinguishing MEM from CA, but this marker appears to be valueless in discriminating MEM from OA.

关键词： malignant mesothelioma metastatic adenocarcinoma immunophenotyping HBME-1 E-cadherin CA19-9 MOC-31 CK7

immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts

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World Journal of Stem Cells 2011年第11期3卷 104-112页

作者： Nesrine Ben Azouna Faouzi Jenhani Lamia Berraeis Zohra Regaya Immunology Research Unit Faculty of Pharmacy of Monastir Monsatir University TU-5000 Monastir Tunisia Cell Im-munology Cytometry and Cell Therapy Laboratories (National Tunisian Blood Center) BP 294 EL Manar II Tunis 2092 Tunisia

AIM:To study the immunophenotype of hematopoietic progenitor cells from cord blood (CB) grafts (n = 39) in comparison with adult apheresis grafts (AG, n = 229) and pre-apheresis peripheral blood (PAPB) samples (n = 908) using flow cytometry analysis.METHODS: First, we performed a qualitative analysis of CD34+ cell sub-populations in both CB and PAPB grafts using the standardized ISHAGE protocol and a wide panel of 20 monoclonal antibodies. Next, we stud-ied some parameters, such as the age of mothers and the weight of newborns, which can influence the qual-ity and the quantity of CD34+ cells from CB. RESULTS: We found that the percentage of apoptotic cells was high in CB in comparison to PAPB (PAPB: 4.6% ± 2.6% vs CB: 53.4% ± 5.2%, P < 0.001). In CB, the weight of newborn and the age of the mother have the influence on CD34+ cells. The follow-up of Ag CD133in the ISHAGE double platform protocol in association with CD45, CD34 and the 7’AAD shows an equal rate between the two cell populations CD133+CD45+CD34+ high and CD34+CD45+ high with a higher percentage. So, is the inclusion of Ac CD133 necessary in the pres-ent panel included in the ISHAGE methodflLast part, we showed a signif icant presence of interferon γ in CB in comparison to PAPB, the annexin showing the high number of apoptotic cells in CB. CONCLUSION: This study demonstrates that many different obstetric factors must be taken into account when processing and cryo-banking umbilical CB units for transplantation.

关键词： immunophenotyping Hematopoietic pro- genitor Cord blood Mobilized blood

Association of immunophenotypic characterization of peripheral lymphocytes with different clinical phenotypes of tuberculosis in Chinese Han children

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Chinese Medical Journal 2012年第2期125卷 275-280页

作者： Xiao Jing Sun Lin Wu Xi-rong Miao Qing Jiao Wei-wei Shen Chen Shen Dan Feng Wei-xing Liu Fang Shen A-dong Capital Med Univ Beijing Childrens Hosp Beijing Pediat Res Inst Minist EducKey Lab Major Dis Children Beijing 100045 Peoples R China Capital Med Univ Beijing Childrens Hosp Beijing Pediat Res Inst Minist EducNatl Key Discipline Pediat Beijing 100045 Peoples R China

Background Very few researchers have studied the changes in peripheral lymphocyte patterns in adult tuberculosis （TB） and even less researches have been conducted in pediatric TB.In this study,we obtained blood samples from 114 Chinese pediatric TB patients and 116 matched controls to study the association of phenotypic subsets of peripheral lymphocytes with different clinical phenotypes of TB.Methods The subjects were classified as the control group and the TB patients group which were further divided into a pulmonary TB group and an extra-pulmonary TB group （more serious than the former）.The distribution of lymphocyte subpopulations,including T lymphocytes,CD4＋ T lymphocytes,CD8＋ T lymphocytes,B lymphocytes,and natural killer （NK） cells,were quantitatively analyzed by flow cytometry.Results Compared to the healthy controls,TB infection was associated with significantly higher B cell （P 〈0.0001）,and lower T cell （P=0.029） and NK cell （P 〈0.0001） percentages.Compared to pulmonary TB patients,extra-pulmonary TB was associated with relatively higher B cell （P=0.073）,and lower T cell percentages （P=-0.021）,higher purified protein derivative （PPD） negative rate （P=-0.061）,and poorer PPD response （P=-0.010）.Most pulmonary TB cases were primary pulmonary TB （89.1%）,and most extra-pulmonary TB cases had TB meningitis （72.1%）.Conclusions This study demonstrates changes in the lymhocyte distribution in children suffering from different clinical phenotypes of TB; such as primary pulmonary TB,and TB meningitis.These patterns may have significance in understanding the pathogenesis and prognostic markers of the disease,and for developing immunomodulatory modalities of therapy.

关键词： child immunophenotyping lymphocytes meningitis tuberculosis

Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta

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World Journal of Gastroenterology 2018年第42期24卷 4787-4797页

作者： Garett Dunsmore Petya Koleva Reed Taylor Sutton Lindsy Ambrosio Vivian Huang Shokrollah Elahi Department of Dentistry and Department of Medical Microbiology and Immunology University of Alberta Department of Dentistry University of Alberta Division of Gastroenterology University of Alberta Division of Gastroenterology University of TorontoMount Sinai HospitalSinai Health System

AIM To understand the effects of delivery mode on the immune cells frequency and function in cord blood and placenta.METHODS We evaluated immunological differences in cord blood and placental tissues for a case of twins one of which delivered vaginally while the other delivered by caesarian section(C-section). Cord blood mononuclear cells were isolated and placenta tissues were processed for cell isolation. Immune phenotyping was performed by flow cytometry methods following staining for T cells, natural killer(NK) cells, monocytes, neutrophils and CD71^+erythroid cells in both cord blood and placenta tissues. In addition, fetal calprotectin of twins was measured 12 wk after birth.RESULTS We found lower percentages of immune cells(e.g. T cells, monocytes and neutrophils) in the cord blood of C-section delivered compared to vaginally delivered newborn. In contrast, percentages of monocytes and neutrophils were > 2 folds higher in the placental tissues of C-section delivered newborn. More importantly, we observed lower percentages of CD71^+ erythroid cells in both cord blood and placental tissues of C-section delivered case. Lower CD71^+ erythroid cells were associated with a more pro-inflammatory milieu at the fetomaternal interface reflected by higher expression of inhibitory receptors on CD4^+ T cells, higher frequency of monocytes and neutrophils. Furthermore, type of delivery impacted the gene expression profile in CD71^+erythroid cells. Finally, we found that C-section delivered child had > 20-fold higher FCP in his fecal sample at 12 wk of age.CONCLUSION Mode of delivery impacted immune cells profile in cord blood/placenta. In particular frequency of immunosuppressive CD71^+ erythroid cells was reduced in C-section delivered newborn.

关键词： Caesarian section Twins Vaginal delivery immunophenotyping CD71+erythroid cells Cord blood Placental tissues

High frequency of CD34+CD38-/low immature leukemia cells is correlated with unfavorable prognosis in acute myeloid leukemia

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World Journal of Stem Cells 2017年第12期9卷 227-234页

作者： Adriana Plesa Charles Dumontet Eve Mattei Ines Tagoug Sandrine Hayette Pierre Sujobert Isabelle Tigaud Marie Pierre Pages Youcef Chelghoum Fiorenza Baracco Helene Labussierre Sophie Ducastelle Etienne Paubelle Franck Emmanuel Nicolini Mohamed Elhamri Lydia Campos Claudiu Plesa Stéphane Morisset Gilles Salles Yves Bertrand Mauricette Michallet Xavier Thomas Laboratory of Hematology Lyon-Sud HospitalHospices Civils de Lyon CRCL INSERM 1052/CNRS 5286 Department of Hematology Hospices Civils de Lyon Laboratory of Hematology Nord Hospital Statistical and Clinical Research Lyon-Sud HospitalHospices Civils de Lyon Department of Pediatric Hematology and BMT IHOP Lyon

AIM To evaluate the importance of the CD34+CD38-cell population when compared to the CD34+CD38+/low and CD34+CD38+/high leukemic cell sub-populations and to determine its correlations with leukemia characteristics and known prognostic factors, as well as with response to therapy and survival.METHODS Two hundred bone marrow samples were obtained at diagnosis from 200 consecutive patients with newly diagnosed acute myeloid leukemia(AML) were studied between September 2008 and December 2010 at our Institution(Hematology Department, Lyon, France). The CD34/CD38 cell profile was analyzed by multiparameter flowcytometry approach using 8 C panels and FACS CANTO and Diva software(BD Bioscience).RESULTS We analyzed CD34 and CD38 expression in bone marrow samples of 200 AML patients at diagnosis, and investigated the prognostic value of the most immature CD34+CD38-population. Using a cut-off value of 1% of CD34+CD38-from total "bulk leukemic cells" we found that a high(> 1%) level of CD34+CD38-blasts at diagnosis was correlated with advanced age, adverse cytogenetics as well as with a lower rate of complete response after induction and shorter disease-free survival. In a multivariate analysis considering age, leukocytosis, the % of CD34+ blasts cells and the standardized cytogenetic and molecular risk subgroups, a percentage of CD34+CD38-leukemic cells > 1% was an independent predictor of DFS [HR = 2.8(1.02-7.73), P = 0.04] and OS [HR = 2.65(1.09-6.43), P = 0.03].CONCLUSION Taken together, these results show that a CD34/CD38 "backbone" for leukemic cell analysis by multicolour flowcytometry at diagnosis provides useful prognostic information.

关键词： CD34+CD38-/low immunophenotyping Leukemic stem cells Acute myeloid leukemia Prognosis

Immunophenotype of myeloid cells in myelodysplastic syndromes and its clinical implications

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Chinese Medical Journal 1998年第1期111卷 28-31页

作者：邵宗鸿陈桂彬林泽嬉张益枝郝玉书储榆林郑以州钱林生杨天楹杨崇礼冯宝章 Institute of Haematology CAMS and PUMC Tianjin 300020 China

OBJECTIVE: To explore the immunophenotype of myeloid cells in myelodysplastic syndyomes (MDS) and its clinical implications. METHODS: A panel of monoclonal antibody was used to detect CD13+, CD33+, CD15+ and CD14+ antigens on the membrane surfaces of myeloid cells in the bone marrow from 51 MDS, 21 aplastic anemia (AA), 21 paroxysmal nocturnal hemoglobinuria (PNH) patients. 10 acute myeloblastic leukemia (AML) patients and 15 normal subjects by immunoenzymatic assay. The morphology and chromosome karyotype of bone marrow cells of MDS patients were also examined. RESULTS: CD14+, CD13+ and CD33+ cells in the bone marrow were more in MDS patients than in normal controls, AA patients and PNH patients. CD15+ cells in the bone marrow were less in MDS patients than in normal controls. CONCLUSIONS: The percentages of CD14+, CD13+ and CD33+ positive cells in the bone marrow of MDS patients were related to the percentage of myeloblasts, the chromosomal aberrations and the response to treatment. It indicated that there is immunophenotypic misexpression of myeloid cells in MDS patients. Immunophenotype analysis of myeloid cells might be useful for the diagnosis and treatment of MDS patients.

关键词： Adolescent Adult Aged Antigens, CD Antigens, CD13 Antigens, CD14 Antigens, Differentiation, Myelomonocytic Bone Marrow Cells Female Humans immunophenotyping Male Middle Aged Myelodysplastic Syndromes Prognosis

Immunophenotype and Ultrastructure of B-cell Lymphoproliferative Disorder with Cytoplasmic Projection

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第3期23卷 236-238页

作者：刘新月胡闲石樊丽华阮幼冰 Institute of Hematology Ultrastructure Department

To identify the knowledge of rare lymphoproliferative disorder, the clinical and biological features of three kinds of lymphoproliferative disorders with cytoplasmic projections were compared The clinical manifestations, ultrastructure and immunophenotype were analyzed The results showed that hairy cell leukemia (HCL), splenic lymphoma with villous lymphocyte (SLVL) and hairy cell leukemia-variant (HCL-V) had some common characters including splenomegaly, peripheral blood and bone marrow infiltration by villous lymphocyte and B lymphocyte immunophenotype; but these three disorders had specific features respectively It was concluded that overall analysis of clinical and laboratory features might be contributive to the differential diagnosis of these three disorders

关键词： lymphoproliferative disorder hairy cell immunophenotyping electron microscopy