Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens
作者机构：Department of Chemical and Biomolecular EngineeringSogang UniversitySeoul 04107Korea Berkeley Sensor and Actuator CenterDepartments of BioengineeringElectrical Engineering and Computer ScienceBiophysics Graduate ProgramUniversity of CaliforniaBerkeleyBerkeleyCA 94720USA Department of Mechanical EngineeringSogang UniversitySeoul 04107Korea
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2018年第7卷第1期
页 面：359-367页
核心收录：
学科分类：0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0702[理学-物理学]
基 金：supported by the Air Force Office of Scientific Research Grants AFOSR FA2386-13-1-4120 by the International Research and Development Program of the National Research Foundation of Korea(NRF) by the Ministry of Science,ICT,and Future Planning(MSIP)(no.2016K1A3A1A32913356) by the Mid-Career Researcher Support Program of NRF by the MSIP(no.2016R1A2B3014157) by the Basic Science Research Program through the NRF funded by the Ministry of Education(no.2016R1A6A1A03012845) by the C1 Gas Refinery Program of NRF by the MSIP(no.2016M3D3A1A01913546) by the Leading Foreign Research Institute Recruitment Program through NRF by the MSIP(no.2013K1A4A3055268) by the National Institutes of Health(NIH)(R01 AI117064-01)
摘 要：A rapid,precise method for identifying waterborne pathogens is critically needed for effective disinfection and better ***,conventional methods,such as culture-based counting,generally suffer from slow detection times and low ***,we developed a rapid detection method for tracing waterborne pathogens by an innovative optofluidic platform,a plasmonic bacteria on a nanoporous mirror,that allows effective hydrodynamic cell trapping,enrichment of pathogens,and optical signal *** designed and simulated the integrated optofluidic platform to maximize the enrichment of the bacteria and to align bacteria on the nanopores and plasmonic mirror via hydrodynamic cell *** nanoparticles are self-assembled to form antenna arrays on the surface of bacteria,such as Escherichia coli and Pseudomonas aeruginosa,by replacing citrate with hydroxylamine hydrochloride in order to amplify the signal of the plasmonic optical *** to the synergistic contributions of focused light via the nanopore geometry,self-assembled nanoplasmonic optical antennas on the surface of bacteria,and plasmonic mirror,we obtain a sensitivity of detecting *** as low as 102 cells/ml via surface-enhanced Raman *** believe that our label-free strategy via an integrated optofluidic platform will pave the way for the rapid,precise identification of various pathogens.
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