Proteomic analysis of heat stress resistance of cucumber leaves when grafted onto Momordica rootstock

作     者：Ye Xu Yinghui Yuan Nanshan Du Yu Wang Sheng Shu Jin Sun Shirong Guo 

作者机构：Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of AgricultureCollege of HorticultureNanjing Agricultural UniversityNanjingChina Department of HorticultureHenan Agricultural UniversityZhengzhouChina Suqian Academy of Protected HorticultureNanjing Agricultural UniversitySuqianChina 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2018年第5卷第1期

页      面：218-235页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：This work was financially supported by the National Natural Science Foundation of China(No.31672199,No.31471869,and No.31401919) the China Agriculture Research System(CARS-23-B12) the Central Research Institutes of Basic Research Fund(KYZ201738) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) 

主　　题：crops stress resistance 

摘      要：Various biotic and abiotic stresses threaten the cultivation of future agricultural *** these stresses,heat stress is a major abiotic stress that substantially reduces agricultural *** strategies to enhance heat stress tolerance of crops have been developed,among which is ***,we show that Momordica-grafted cucumber scions have intrinsically enhanced chlorophyll content,leaf area,and net photosynthetic rate under heat stress compared to plants grafted onto cucumber *** investigate the mechanisms by which Momordica rootstock enhanced cucumber scions heat stress tolerance,comparative proteomic analysis of cucumber leaves in response to rootstock-grafting and/or heat stress was ***-seven differentially accumulated proteins involved in diverse biological processes were identified by two-dimensional electrophoresis(2-DE)in conjunction with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry(MALDI-TOF/TOF MS).The following four main categories of proteins were involved:photosynthesis(42.8%),energy and metabolism(18.2%),defense response(14.3%),and protein and nucleic acid biosynthesis(11.7%).Proteomic analysis revealed that scions grafted onto Momordica rootstocks upregulated more proteins involved in photosynthesis compared to scions grafted onto cucumber rootstocks under heat stress and indicated enhanced photosynthetic capacity when seedlings were exposed to heat ***,the expression of photosynthesis-related genes in plants grafted onto Momordica rootstocks significantly increased in response to heat *** addition,increased high-temperature tolerance of plants grafted onto Momordica rootstock was associated with the accumulation of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and oxygen-evolving enhancer protein 1(OEE1).Taken together,the data indicated that Momordica rootstock might alleviate growth inhibition caused by heat stress by improving ph