Protection of cadmium chloride induced DNA damage by Lamiaceae plants

作     者：Ramaraj Thirugnanasampandan Rajarajeswaran Jayakumar 

作者机构：PG and Research Department of BiotechnologyKongunadu ArtsScience CollegeGN Mills Department of ZoologyBharathiar University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2011年第1卷第5期

页      面：391-394页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by PG and Research Department of Biotechnology.Kongunadu Arts and Science College.GN Mills.India 

主　　题：Cadmium chloride DNA damage Lamiaceae plants Phenols Comet assay Antioxidant activity 

摘      要：Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica(A.malabarica),Leucas aspera(L.aspera)and Ocimum basilicum(0.basilicum).Methods:The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants.To determine the DNA protecting activity,various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells.The pretreated cells were exposed to H_2O_2to induce DNA damage through oxidative stress.Comet assay was done and the tail length of individual comets was measured.Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.Results:Among the three plant extracts,the highest amount of total phenolic content was found in O.basilicum(189.33 mg/g),whereas A.malabarica showed high levels of flavonoids(10.66 mg/g).O.basilicum also showed high levels of DNA protecting(85%)and radical scavenging activity.Conclusions:The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage.