MiRNA profile in esophageal squamous cell carcinoma:Downregulation of miR-143 and miR-145
MiRNA profile in esophageal squamous cell carcinoma:Downregulation of miR-143 and miR-145作者机构：Department of Biochemistry and Molecular Biology Medical College of Shantou University Shantou 515041 Guangdong Province China Institute of Oncologic Pathology Medical College of Shantou University Shantou 515041 Guangdong Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第1期
页 面：79-88页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：Supported by Grants from the NSFC-Guangdong Joint Fund,No.U0932001 the National Natural Science Foundation of China,No.30900560
主 题：Esophageal squamous cell carcinoma MicroRNA miR-143 miR-145 Tumor invasion depth
摘 要：AIM:To investigate the expression profile of miRNA in esophageal squamous cell carcinoma(ESCC).METHODS:The expression profile of miRNA in ESCC tissues was analyzed by miRNA *** expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction(PCR) using TaqMan *** mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression ***:A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues *** miR-143 and miR-145 expression correlated with tumor invasion *** transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant ***:These findings suggest that the deregulation of miRNAs plays an important role in the progression of *** miR-143 and miR-145 might act as anti-oncomirs common to ESCC.
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