Ischemic preconditioning in immature hearts: mechanism and compatibility with cardioplegia

作     者：朱斌 闵苏 龙村 叶铁虎 

作者机构：Department of AnesthesiologyPeking Union Medical HospitalCAMS&PUMC Department of AnesthesiologyFirst HospitalChongqing Medical University Anesthesiology & CPB Research GroupFuwai HospitalCAMS & PUMC 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第2期

页      面：253-257页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

主　　题：ischemic preconditioning immature K A TP channels ischemia cardioplegia 

摘      要：To investigate (1) whether ischemic preconditioning (IPC) could protect immature rabbit hearts against ischemia reperfusion injury and (2) the role of K ATP channel in the mechanism of myocardial protection Since cardioplegia is a t raditional and effective cardioprotective measure in clinic, our study is also d esigned to probe the compatibility between IPC and cardioplegia Methods New Zealand rabbits aged 14-21 days weighing 220-280 g were used The animals w ere anesthetized and heparinized The chest was opened and the heart was quickl y removed for connection of the aorta via Langendorff s method within 30 s after excision All hearts were perfused with Krebs Henseleit buffer balanced with gas mixture (O 2∶CO 2=95%∶5%) at 60 cm H 2O (perfusion pressure) IPC cons isted of 5 min global ischemia plus 10 min reperfusion Glibenclamide was used as the K ATP channel blocker at a concentration of 10 μmol/L before IPC Cardiac arrest was induced with 4℃ St Thomas cardioplegic solution, at which point the heart was made globally ischemic by withholding perfusion for 45 min f ollowed by 40 min reperfusion Thirty immature rabbit hearts were randomly divi ded into four groups: CON (n=9) was subjected to ischemia reperfusion only; IPC ( n=9) underwent IPC and ischemia reperfusion; Gli (n=6) was given glibenclamide and ischemia reperfusion; and Gli+IPC (n=6) underwent glibenclamide, IPC and is chemia reperfusion Coronary flow (CF), HR, left ventricle developed pressure (LVDP), and ±dp/dt max were monitored at equilibration (baseline value) an d 5, 1 0, 20, 30 and 40 min after reperfusion The values resulting from reperfusion w ere expressed as a percentage of their baseline values Arrhythmia quantificati on, myocardial enzyme in the coronary effluent and myocardial energy metaboli sm were also determined Results The recovery of CF, HR, LVDP and ±dp/dt max in preconditioned hearts was b est am ong the four groups