咨询与建议

看过本文的还看了

相关文献

该作者的其他文献

文献详情 >Function coupling of otoferlin... 收藏

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity

作     者:Wu Wu Mona N.Rahman Jun Guo Natalie Roy Lihua Xue Catherine M.Cahill Shetuan Zhang Zongchao Jia 

作者机构:Department of Biomedical and Molecular SciencesQueen’s UniversityKingstonOntario K7L 3N6Canada Department of Anesthesiology&Perioperative CareUniversity of California IrvineIrvineCAUSA 

出 版 物:《分子细胞生物学报:英文版》 (Journal of Molecular Cell Biology)

年 卷 期:2015年第7卷第2期

页      面:168-179页

核心收录:

学科分类:1002[医学-临床医学] 10[医学] 

基  金:This work was supported by funding from Canadian Institutes of Health Research and Natural Sciences and Engineering Research Council of Canada.Z.J.is a Canada Research Chair in Structural Biology 

主  题:otoferlin GAD65 GABA neurotransmitter exocytosis 

摘      要:Otoferlin,an integral membrane protein implicated in a late stage of exocytosis,has been reported to play a critical role in hearing although the underlying mechanisms remain ***,its widespread tissue distribution infers a more ubiquitous role in synaptic vesicle ***,an excitatory neurotransmitter,is converted to its inhibitory counterpart,g-aminobutyric acid(GABA),by L-glutamic acid decarboxylase(GAD),which exists in soluble(GAD67)and membrane-bound(GAD65)*** the first time,we have revealed a close association between otoferlin and GAD65 in both HEK293 and neuronal cells,including SHSY5Y neuroblastoma and primary rat hippocampus cells,showing a direct interaction between GAD65 and otoferlin’s C2 *** primary rat hippocampus cells,otoferlin and GAD65 co-localized in a punctate pattern within the cell body,as well as in the axon along the path of vesicular ***,GABA is virtually abolished in otoferlin-knockdown neuronal cells whereas otoferlin overexpression markedly increases endogenous *** attenuation in otoferlin-knockdown primary cells is correlated with diminished L-type calcium *** previously unknown and close correlation demonstrates that otoferlin,through GAD65,modulates GABAergic *** discovery of otoferlin–GAD65 functional coupling provides a newavenue for understanding the molecular mechanism by which otoferlin functions in neurological pathways.

读者评论 与其他读者分享你的观点

用户名:未登录
我的评分