Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

作     者：ZHANG Zhi-bang XU Lei WEN Xue-xia DONG Jian-guo ZHOU Lei GE Xin-na YANG Han-chun GUO Xin 

作者机构：Key Laboratory of Animal Epidemiology and ZoonosisMinistry of Agriculture/College of Veterinary MedicineChina Agricultural University/State Key Laboratory of AgrobiotechnologyChina Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2018年第17卷第5期

页      面：1171-1180页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Key Technology R&D Program of China (2015BAD12B01-2) the Major Program of National Natural Science Foundation of China (31490603) the earmarked fund for Modern Agroindustry Technology Research System of China (CARS-36) 

主　　题：porcine reproductive and respiratory syndrome virus(PRRSV) nonstructural protein 10(nsp10) viral replication 

摘      要：The nonstructural protein 10（nsp10） of porcine reproductive and respiratory syndrome virus（PRRSV） encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10 a, was found in PRRSV-infected cells and the production of nsp10 a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10 a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10 a production. Finally, we demonstrated that nsp10 a exerted little influence on the growth kinetics of PRRSV in vitro.