Evaluation of Circulating Endometrial Cells as a Biomarker for Endometriosis

作     者：Ying Chen Hong-Lan Zhu Zhe-Wen Tang Kuang Hong Neoh Dong-Fang Ouyang Heng Cui Hong-Yan Cheng Rui-Qiong Ma Xue Ye Ray P. S. Han Xiao-Hong Chang Chen Ying;Zhu Hong-Lan;Tang Zhe-Wen;Neoh Kuang Hong;Ouyang Dong-Fang;Cui Heng;Cheng Hong-Yan;Ma Rui-Qiong;Ye Xue;Han Ray P. S.;Chang Xiao-Hong

作者机构：Gynecological Oncology Center Peking University People's Hospital Beijing 100044 China Department of Materials Science and Engineering Peking University Beijing 100871 China Department of Mechanical and Industrial Engineering University of Toronto Toronto Ontario M5S 3G8 Canada 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2017年第130卷第19期

页      面：2339-2345页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：国家自然科学基金 the Peking University People's Hospital Scientific Research Development Funds 

主　　题：Biomarker Circulating Endometrial Cells Clinical Diagnosis Endometriosis Pathogenesis 

摘      要：Background： Circulating endometrial cells （CECs） have been reported to be present in the peripheral blood of women with endometriosis （EM）, providing clear and specific evidence of the presence of ectopic lesions. In this study, we established a method with a high detection rate of CECs, assessed the diagnostic value of CECs for EM and compared with serum CA125, and proposed a hypothesis for the pathogenesis of EM from the new perspective of CECs. Methods： The participants were enrolled prospectively from October 2015 to July 2016. The peripheral blood samples were collected from 59 participants, and the blood cells were isolated for immunofluorescence staining via microfluidic chips. The cells that were positive for vimentin/cytokeratin and estrogen/progesterone receptor and negative for CD45 were identified as CECs. The serum CA125 level was tested with electrochemiluminescence immunoassay. Results： The detection rate of CECs reached 89.5% （17/19） in the EM group, which was significantly higher than that of the control group （15.0% [6/40], P 〈 0.001） and was independent of menstrual cycle phases. Furthermore, a positive CEC assay detected 4/5 cases of Stage Ⅰ–Ⅱ EM. In contrast, a positive CA125 test had limited value in detecting EM （13/19, 68.4%） and detected only one case of Stage Ⅰ–Ⅱ EM. Conclusion： CECs are promising biomarkers for EM with great potential for a noninvasive diagnostic assay.