采用手持式光度计的△I测量模式快速测定尿样中白蛋白
Rapid Determination of Albumin of Urine in ΔI Measurement Mode with Portable Spectrophotometer of High Sensitivity作者机构:吉林大学化学系 吉林大学分子酶学工程教育部重点实验室长春130023
出 版 物:《高等学校化学学报》 (Chemical Journal of Chinese Universities)
年 卷 期:2001年第22卷第6期
页 面:928-930页
核心收录:
学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术(可授医学、理学学位)] 070302[理学-分析化学] 0703[理学-化学] 10[医学]
基 金:吉林省科学技术厅! (批准号 :吉科合字第 2 0 0 0 0 10 5号 )资助
主 题:手持式光度计 △I测量模式 白蛋白 尿样 测定 临床检验 光度法
摘 要:A portable spectrophotometer developed by Changchun R & D Center for Analytical Instruments, which combines ΔI measurement mode and a measurement mode together with a high sensitivity, good accuracy and low cost, is employed to replace the traditional spectrophotometer and the sensitivity is enhanced. Therefore, a new rapid method with low cost and simplicity in operation for protein determination is developed. For this method, the linear range is shown to be 0—0 74 μg/mL, the detection limit is 0 01 μg/mL, and the analysis can be completed within 5 min. The albumin in urine is determined and all the results are confirmed by the standard method. Because of the rapid analysis speed, less expenditure, more convenience and simplicity in operation of this method, it is possible to use this method to diagnose glycosuric nephropathy at an early stage, even do it at home.