AP-2α expression and cell apoptosis of the lung tissue of rats with COPD and ECV304 cells stimulated by cigarette smoke extract

作     者：LI JunLi CHEN Yan CHEN Ping CAI Shan PENG Hong ZHOU Rui XIANG XuDong LONG Hong LIU ShaoKun 

作者机构：Department of Respiratory Medicine Xiangya Second Hospital of Central South University Changsha Hunan 410011 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2011年第56卷第15期

页      面：1562-1568页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported in part by the National Natural Science Foundation of China (30770931 81070039) 

主　　题：慢性阻塞性肺病 细胞凋亡 香烟烟雾 提取物 AP 大鼠 caspase 癌组织 

摘      要：An increasing body of evidence suggests that apoptosis of structural cells in the lung might be an important upstream event in the pathogenesis of chronic obstructive pulmonary disease (COPD).AP-2α is one of the important transcription factors involved in the modulation of apoptosis in carcinogenesis and idiopathic-dilated *** relationship between AP-2α and apoptosis in COPD remains to be *** aim of the present study was to investigate the expression of AP-2α in the lung tissues of rats with COPD induced by smoking and its possible protective effect on cigarette smoke extract (CSE) induced endothelial cell ***-Dawley rats (n=24) were randomly assigned to normal and COPD *** COPD group was exposed to smoke from 20 commercial unfiltered cigarettes for 80 d before morphological assessment of the lung tissue was *** expression of AP-2α in lung tissues was measured by Western *** demonstrate the relationship between apoptosis and AP-2α,in vitro cell experiments were carried *** were treated with different concentrations of CSE before proliferation was measured by *** was then determined by Hoechst staining and the expression of cleaved caspase-3 and AP-2α by Western blotting over time following treatment with 5% *** were then infected with an AP-2α adenovirus vector and the expression of cleaved caspase-3 and AP-2α was compared to the control groups by Western *** COPD group showed larger air spaces and significant decrease of FEV 0.3/FVC compared with the rats in the control group (P0.05).The expression of AP-2α was significantly higher in the lung tissue of rats with COPD compared with those of controls (P0.05).In the ECV304 cells,CSE induced apoptosis (P0.01) and caspase-3 activation in a time-dependent manner and reduced the cell proliferation rate in a dose-dependent manner (P0.005).Moreover,5% CSE treatment increased endogenous AP-2α protein ***-2α overexpr