Immunohistochemical evaluation of vitamin D receptor(VDR) expression in cutaneous melanoma tissues and four VDR gene polymorphisms

作     者：Francesco La Marra Giuseppe Stinco Cinzia Buligan Giovanni Chiriacò Diego Serraino Carla Di Loreto Sabina Cauci 

作者机构：Department of Medical AreaSchool of MedicineUniversity of Udine Dermatology ClinicUdine University-HospitalUniversity of Udine Unit of Cancer EpidemiologyCRO Aviano National Cancer Institute 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2017年第14卷第2期

页      面：162-175页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：维生素D受体 基因多态性 黑色素瘤 免疫组织 VDR 皮肤癌 化学研究 限制性片段长度多态性 

摘      要：Objective:Vitamin D receptor(VDR)mediates vitamin D *** examined whether VDR expression in excised melanoma tissues is associated with VDR gene(VDR)***:We evaluated VDR protein expression(by monoclonal antibody immunostaining),melanoma characteristics,and carriage of VDR-Fok I-rs2228570(CT),VDR-Bsm I-rs1544410(GA),VDR-ApaI-rs7975232(TG),and VDR-TaqI-rs731236(TC)polymorphisms(by restriction fragment length polymorphism).Absence or presence of restriction site was denoted by a capital or lower letter,respectively:Fandffor Fok I,Bandbfor Bsm I,Aandafor ApaI,and Tandtfor TaqI ***-four Italian cutaneous primary melanomas(52.1±12.7 years old)were studied;51.4% were stage Ⅰ,21.6% stage Ⅱ ,13.5% stage Ⅲ,and 13.5% stage Ⅳ *** expression was categorized as follows:100% positive vs.1,regression,tumor-infiltrating lymphocytes,tumoral infiltration of vascular tissues,additional skin and non-skin cancers,and melanoma ***:We highlighted that VDR polymorphisms can affect VDR expression in excised melanoma *** VDR expression in AATT carriers is a new finding that merits further *** expression possibly poses implications for vitamin D supplementation against *** expression and VDR genotype may become precise medicinal tools for melanoma in the future.