Estrogen up-regulates MMP2/9 expression in endometrial epithelial cell via VEGF-ERK1/2 pathway
Estrogen up-regulates MMP2/9 expression in endometrial epithelial cell via VEGF-ERK1/2 pathway作者机构：Department of Gynaecology and Obstetrics of Hainan People's Hospital
出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)
年 卷 期：2013年第6卷第10期
页 面：826-830页
核心收录：
学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学]
基 金：supported by Natural Science Foundation of Hainan Province(No.812148)
主 题：Dysfunctional uterine bleeding Matrix metalloproteinase 2 and 9 Vascular endothelial growth factor ERK1/2 signal pathway Estrogen Primary uterine epithelial cells
摘 要：Objective:To study the effect of estrogen on anovulatory dysfunctional uterine bleeding(ADUB).Methods:Primary endometrial epithelial cells of Hainan Lizu female was cultured and hydrolylic activity of gelalinase was determined by gelatin zymography *** mRNA and protein synthesis was blocked respectively to determine whether the increased expression of MMP-2/9 was induced by *** expression of VEGF was blocked by *** treatment with various ***-9,VEGF,total Erk and phosphorylated Erk expression in primary uterine epithelial cells was detected by Western blotting *** MMP-2/9mRNA levels was measured by real-time ***:The activity and expression of MMP2/9 was inereased in the endometrium of patients with *** could up-regulate the expression of VEGF and activate Erk 1/2-Elk1 signal *** interference by siRNA,ERK1/2 pathway was blocked in cells,and the expression of MMP-2/9 was ***1/2 specific blocker U0126 blocked ERK phosphorylation,and it could down-regulate the expression of MMP-2/***:The results showed that the estrogen can increase the expression of VEGF,and thus activate ERK1/2 pathway to induce MMP-2/9 expression.
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