Metabolite changes in the ipsilateral and contralateral cerebral hemispheres in rats with middle cerebral artery occlusion
Metabolite changes in the ipsilateral and contralateral cerebral hemispheres in rats with middle cerebral artery occlusion作者机构：Department of Radiology First Affiliated Hospital of Wenzhou Medical University Department of Radiology First Affiliated Hospital of Zhengzhou University School of Pharmacy Wenzhou Medical University
出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)
年 卷 期：2017年第12卷第6期
页 面：931-937页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学]
基 金：supported by grants from the Health Innovation Talents Project of Zhejiang Province of China,No.2016 the National Natural Science Foundation of China,No.81571626,U1404823 the Natural Science Foundation of Zhejiang Province of China,No.LY15H220001 the Medical and Health Research Project of Zhejiang Province of China,No.2014KYA134 the Wenzhou Bureau of Science and Technology of China,No.Y20140731,Y20150087
主 题：occlusion hemisphere glutamate aspartate metabolite creatinine lactate neuronal glycine carotid
摘 要：Cerebral ischemia not only causes pathological changes in the ischemic areas but also induces a series of secondary changes in more distal brain regions（such as the contralateral cerebral hemisphere）. The impact of supratentorial lesions, which are the most common type of lesion, on the contralateral cerebellum has been studied in patients by positron emission tomography, single photon emission computed tomography, magnetic resonance imaging and diffusion tensor imaging. In the present study, we investigated metabolite changes in the contralateral cerebral hemisphere after supratentorial unilateral ischemia using nuclear magnetic resonance spectroscopy-based metabonomics. The permanent middle cerebral artery occlusion model of ischemic stroke was established in rats. Rats were randomly divided into the middle cerebral artery occlusion 1-, 3-, 9-and 24-hour groups and the sham group. ~1H nuclear magnetic resonance spectroscopy was used to detect metabolites in the left and right cerebral hemispheres. Compared with the sham group, the concentrations of lactate, alanine, γ-aminobutyric acid, choline and glycine in the ischemic cerebral hemisphere were increased in the acute stage, while the concentrations of N-acetyl aspartate, creatinine, glutamate and aspartate were decreased. This demonstrates that there is an upregulation of anaerobic glycolysis（shown by the increase in lactate）, a perturbation of choline metabolism（suggested by the increase in choline）, neuronal cell damage（shown by the decrease in N-acetyl aspartate） and neurotransmitter imbalance（evidenced by the increase in γ-aminobutyric acid and glycine and by the decrease in glutamate and aspartate） in the acute stage of cerebral ischemia. In the contralateral hemisphere, the concentrations of lactate, alanine, glycine, choline and aspartate were increased, while the concentrations of γ-aminobutyric acid, glutamate and creatinine were decreased. This suggests that there is a difference in the metabolite chan
维普期刊数据库
同方期刊数据库