Proteomic identification of cryostress in epididymal spermatozoa

作     者：Sung Jae Yoon Md Saidur Rahman Woo Sung Kwon Do Yeal Ryu Yoo Jin Park Myung Geol Pang 

作者机构：Department of Animal Science&TechnologyChung-Ang UniversityAnseongGyeonggi-Do 456-756Republic of Korea 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2017年第8卷第2期

页      面：478-489页

核心收录：

学科分类：08[工学] 0905[农学-畜牧学] 09[农学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：provided by “Cooperative Research Program for Agriculture Science&Technology Development(Project No.PJ01106101)”Rural Development Administration(RDA) 

主　　题：Cryopreservation Cryostress Protein Spermatozoa 

摘      要：Background： Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation,thereby reducing sperm quality. We assessed the motility（%）, motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence ***, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis（2-DE） results using two commercial ***： Cryopreservation reduced viability（%）, motility（%）, straight-line velocity（VSL）, average path velocity（VAP）, amplitude of lateral head displacement（ALH）, and capacitated spermatozoa, whereas straightness（STR）and the acrosome reaction increased after cryopreservation（P 3 fold, P ＆lt; 0.05） before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation,and the guanylate cyclase ***： The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.