应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理

作     者：王瑞勇 蔡汝秀 林智信 

作者机构：武汉大学化学与分子科学学院武汉430072 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：2004年第25卷第2期

页      面：267-269页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：国家自然科学基金 (批准号 :2 0 2 75 0 2 7)资助 

主　　题：氧合血红蛋白 过氧化氢 相互作用 反应动力学 反应机理 停流时间分辨光谱 

摘      要：Interaction between oxyhemoglobin and H2O2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H2O2 suggested the presence of an intermediate species. The influence of pH, concentration of H2O2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.