Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells
Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells作者机构：Centre for Process Systems Engineering Department of Chemical Engineering Imperial College London London UK Department of Life Sciences Imperial College London London UK Centre for Synthetic Biology and Innovation Imperial College London London UK Department of Life Sciences Imperial College London London UK Centre for Synthetic Biology and Innovation Imperial College London London UK Centre for Process Systems Engineering Department of Chemical Engineering Imperial College London London UK
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2013年第4卷第12期
页 面：1013-1019页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Transient Gene Expression Transfection Efficiency Chinese Hamster Ovary Cells mRNA Stability Temperature Shift
摘 要：Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.
维普期刊数据库