Overexpression of S-adenosylmethionine decarboxylase(SAMDC)in Xeno-pus embryos activates maternal program of apoptosis as a "fail-safe"mechanism of early embryogenesis

作     者：MASATAKEKAI,CHIKARAKAITO,HIROSHIFUKAMACHI,TAKAYASUHIGO,EIJI TAKAYAMA,HIROSHI HARA,YOSHIKAZU OHYA,KAZUEI IGARASHI,KOICHIRO SHIOKAWA 

作者机构：Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Department of Parasitology National Defense Medical College Namiki 3-2 Tokorozawa Saitama 359-8843 Japan Medicinal Research Laboratories Taisho Pharmaceutical Co. Ltd. 1-403 Yoshino-cho Kita-ku Saitama 331-9530 Japan Department of Integrated Biosciences Graduate School of Frontier Sciences University of Tokyo 5-1-5 Kashiwanoha Kashiwa Chiba 277-8562 Japan Faculty of Pharmaceutical Sciences Chiba University Yayoi-cho 1-33 Inage-ku Chiba 263-8522 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第3期

页      面：147-158页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

主　　题：SAMDC overexpression maternal apoptosis program processing-defective SAMDC mutant MBT fail-safe mechanism. 

摘      要：In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due toactivation of the maternal program of apoptosis. We injected SAMDC mRNA into only one of the animal side blastomeres of embryos at different stages of cleavage, and examined the timing of the onset of theapoptotic reaction. In the injection at 4- and 8-cell stages, a considerable number of embryos developed intotadpoles and in the injection at 16- and 32-cell stages, all the embryos became tadpoles, although tadpolesobtained were sometimes abnormal. However, using GFP as a lineage tracer, we found that descendant cellsof the blastomere injected with SAMDC mRNA at 8- to 32-cell stages are confined within the blastocoel atthe early gastrula stage and undergo apoptotic cell death within the blastocoel, in spite of the continueddevelopment of the injected embryos. These results indicate that cells overexpress