Pretreated quercetin protects gerbil hippocampal CA1 pyramidal neurons from transient cerebral ischemic injury by increasing the expression of antioxidant enzymes

作     者：Bai Hui Chen Joon Ha Park Ji Hyeon Ahn Jeong Hwi Cho In Hye Kim Jae Chul Lee Moo-Ho Won Choong-Hyun Lee In Koo Hwang Jong-Dai Kim Il Jun Kang Jun Hwi Cho Bich Na Shin Yang Hee Kim Yun Lyul Lee Seung Min Park 

作者机构：Department of Histology and EmbryologyInstitute of NeuroscienceWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Biomedical Science and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea Department of NeurobiologySchool of MedicineKangwon National UniversityChuncheonSouth Korea Department of PharmacyCollege of PharmacyDankook UniversityCheonanSouth Korea Department of Anatomy and Cell BiologyCollege of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea Division of Food BiotechnologySchool of BiotechnologyKangwon National UniversityChuncheonSouth Korea Department of Food Science and NutritionHallym UniversityChuncheonSouth Korea Department of Emergency MedicineSchool of MedicineKangwon National UniversityChuncheonSouth Korea Department of PhysiologyCollege of Medicineand Institute of Neurodegeneration and NeuroregenerationHallym UniversityChuncheonSouth Korea Department of SurgerySchool of MedicineKangwon National UniversityChuncheonSouth Korea Department of Emergency MedicineSacred Heart HospitalCollege of MedicineHallym UniversityAnyangSouth Korea 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2017年第12卷第2期

页      面：220-227页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(NRF-2015R1D1A1A01059728) Hallym University Research Fund 2014(HURF-2014-25) 

主　　题：nerve regeneration flavonoids transient cerebral ischemia Cu/Zn superoxide dismutase catalase Mn superoxide dismutase glutathione peroxidase neural regeneration 

摘      要：Quercetin（QE; 3,5,7,3′,4′-pentahydroxyflavone）, a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.