Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats
Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats作者机构：Department of Biochemistry and Molecular Biology College of Life Sciences Bioactive Materials Key Lab of Ministry of Education Nankai University Tianjin 300071 China Department of Medical Oncology Dana Farber Cancer Institute Harvard Medical School Boston MA 02115 USA Department of Urology Innsbruck Medical University Anichstrasse 35 A-6020 Innsbruck Austria The Department of Urology Northwestern University Feinberg School of Medicine Chicago IL 60611 USA
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2009年第11卷第4期
页 面：451-459页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 071002[理学-动物学]
基 金：Acknowledgment This research was funded by the following grants: the National Basic Research Program (973 Program No.2009CB918900) the National Natural Science Foundation of China (grant No. 30672101 30872592) the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20070055023) and the key research project of Tianjin Municipal Science and Technology Commission (grant No. 06YFSYSF02000 07jczdjc08300). The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work
主 题：androgens castration estrogen prostatic hyperplasia rat stromal cells
摘 要：It is known that human benign prostatic hyperplasia might arise from an estrogen/androgen （E/T） imbalance. We studied the response of castrated rat prostate to different ratios of circulating E/T. The castrated male Wistar rats were randomly injected with E/T at different ratios for 4 weeks. The prostates of E/T （1：100） group showed a distinct prostatic hyperplasia response by prostatic index, hematoxylin and eosin staining, and quantitative immunohistochemical analysis of a-smooth muscle actin （SMA）. In this group, cells positive for Vimentin, non-muscle myosin heavy chain （NMMHC） and proliferating cell nuclear antigen （PCNA） increased in the stroma and epithelium. Furthermore, the mRNA levels of smooth muscle myosin heavy chain （SMMHC） and NMMHC increased. So E/T at a ratio of 1：100 can induce a stromal hyperplastic response in the prostate of castrated rats. The main change observed was an increase of smooth muscle cells, whereas some epithelial changes were also seen in the rat prostates.
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