Effects of Alkaloids in Oxytropis glabra on Mouse T Lymphocytes
Effects of Alkaloids in Oxytropis glabra on Mouse T Lymphocytes作者机构：Linyi Agricultural School Junan 276600 China Laboratory of Animal Clinical Diagnosis Technology of the Ministry of Agriculture College of Veterinary Medicine of Inner Mongolia Agricultural University Huhhot 010018 China Animal Epidemic Prevention and Control Center of Dongsheng District Dongsheng 017000 China
出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)
年 卷 期：2016年第8卷第4期
页 面：211-214页
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：Supported by Natural Science Foundation of Inner Mongolia(2012MS0404)
主 题：lymphocyte Oxytropis suppressed poisoning stimulation alkaloid seriously extremely Mouse doses
摘 要：The effects of different doses of swainonine（ SW）,thermopsine（ Ts） and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 240 mice were randomly grouped,and orally administrated with different concentrations of SW,Ts and their mixture. Four mice were randomly selected from each group at the 7th,14th,21st and 35th days,and lymphocytes were separated from anti-coagulated blood collected from eyeball. After Con A stimulation,T lymphocyte proliferation was detected by MTT assay. The results showed that low dose of SW promoted proliferation of mouse T lymphocyte; medium and high dose of SW also promoted proliferation of mouse T lymphocyte within a short period,but would seriously suppress its proliferation after a long period. Low dose of Ts almost had no impact on proliferation of T lymphocyte,while high dose also suppressed its proliferation.A similar result with SW was observed on alkaloid mixture. This indicated that different doses of SW,Ts and their mixture affected proliferation of T lymphocyte in different degrees.
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