LARGE-SCALE PURIFICATION OF PROTHORACICOTROPIC HORMONE OF THE SILKWORM (BOMBYX MORI)

作     者：长澤宽道 郭郛 钟香臣 夏邦颖 王宗舜 仇序佳 魏定义 陈娥英 王敬泽 铃木昭宪 磯贝彰 堀康宏 田村三郎 石崎宏矩 HIROMICHI NAGASAWA , GUO FU ZHONG XIANGCHEN XIA BANGYING WANG ZONGSHUN QIU XUJIA WEI DINGYI CHEN EYING WANG JINGZE(Institute of Zoology, Academia Sinica) AKINORI SUZUKI AKIRA ISOGAI YASUHIRO HORI SABURO TAMURA(The University of Tokyo, Japan)AND HIRONORI ISHIZAKI(Nagoya University, Japan)

作者机构：Institute of Zoology Academia Sinica The University of Tokyo Japan Nagoya University Japan 

出 版 物：《中国科学A辑(英文版)》 (Science in China,Ser.A)

年 卷 期：1980年第8期

页      面：1053-1060页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：purified Bombyx silkworm crude purification millions ammonium brains partially dissolved 

摘      要： Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7μg of the crude preparation ( crude PTTH ) shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the crude PTTH derived from 100,000 Bombyx heads yields 3.4mg of purified preparation ( highly purified PTTH ), of which 7ng causes adult development in a debrained Samia pupa.