A Surgery Protocol for Adult Zebrafish Spinal Cord Injury

作     者：Ping Fang Jin-Fei Lin Hong-Chao Pan Yan-Qin Shen Melitta Schachner 

作者机构：Center for Neuroscience Shantou University Medical College 22 Xinling Road Shantou 515041 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2012年第39卷第9期

页      面：481-487页

核心收录：

学科分类：0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 071006[理学-神经生物学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.81072622 and 31271580) Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(No. 2010-1561) the Li Kashing Foundation 

主　　题：Zebrafish Spinal cord injury Surgery Locomotor recovery Axonal regrowth 

摘      要：Adult zebrafish has a remarkable capability to recover from spinal cord injury,providing an excellent model for studying neuro-regeneration. Here we list equipment and reagents,and give a detailed protocol for complete transection of the adult zebrafish spinal cord. In this protocol,potential problems and their solutions are described so that the zebrafish spinal cord injury model can be more easily and reproducibly *** addition,two assessments are introduced to monitor the success of the surgery and functional recovery：one test to assess free swimming capability and the other test to assess extent of neuroregeneration by in vivo anterograde axonal *** the swimming behavior test,successful complete spinal cord transection is monitored by the inability of zebrafish to swim freely for 1 week after spinal cord injury,followed by the gradual reacquisition of full locomotor ability within 6 weeks after *** a morphometric correlate,anterograde axonal tracing allows the investigator to monitor the ability of regenerated axons to cross the lesion site and increasingly extend into the gray and white matter with time after injury,confirming functional *** zebrafish model provides a paradigm for recovery from spinal cord injury,enabling the identification of pathways and components of neuroregeneration.