Effect of the P1 Medium and the ECM Medium on Embryo Quality in IVF

作     者：Qian CHEN Ai-jun ZHANG Yun FENG Xiao-wei LU Dong-mei JI Zhi-peng XU Qian CHEN1, Ai-jun ZHANG1, Yun FENG1, Xiao-wei LU1, Dong-mei JI1, Zhi-peng XU2 1. Center of Reproductive Medicine, Ruijing Hospital, Medical School of Shanghai Jiaotong University, Shanghai 200025, China 2. Center of Reproductive Medicine, Drum Tower Hospital, Medical School of Nanjin University, Nanjin 210008, China

作者机构：Center of Reproductive Medicine Ruijing Hospital Medical School of Shanghai Jiaotong University Shanghai200025 China Center of Reproductive Medicine Drum Tower Hospital Medical School of Nanjin University Nanjin 210008 China 

出 版 物：《Journal of Reproduction and Contraception》 (生殖与避孕（英文版）)

年 卷 期：2009年第20卷第2期

页      面：67-72页

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 0905[农学-畜牧学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 090501[农学-动物遗传育种与繁殖] 

主　　题：culture media IVF preimplantation embryo development 

摘      要：Objective To investigate the effect of the glucose-free preimplantation stage one （P1） medium and the ECM medium on embryo development quality in IVF. Methods The patients with ≥4 zygotes of 2PN were studied. A total of 201 retrieval cycles were included in a prospective randomized study. Each patient was herself control. Half of zygotes of 2PN were transferred into ECM medium （group A） and half into P1 medium （group B）for further culture. Embryo development was evaluated on the day of embryo transfer. The efficacy of ECM was compared with P1 as culture medium for the development of preimplantation embryos. Results No statistically significant differences were noted between the two groups regarding embryo-cleavage rate （97.13% vs 97.55%） and rate of normal-cleaving embryos （58.29% and 58.37%）. The rate of top-quality embryos was statistically higher in group A than in group B （27.59% vs 19.75%, P〈O.05）. Embryo quality, as assessed by morphological parameters （the amount of detached anuclear fragments 〉30%）, was better in group A than in group B （19.86% vs 21.75%）, however, there was no statistically significance. Both the rate of good-quality embryos （47.95% vs 46.17%） and available embryos （63.22% vs 61.19%） were higher in group A than in group B, but there was also no statistically significance. Conclusion The ECM medium may be associated with a better embryo quality compared with the P1 medium.