Cloning ＆ sequence identification of Hsp27 gene and expression analysis of the protein on thermal stress in Lucilia cuprina
Cloning ＆ sequence identification of Hsp27 gene and expression analysis of the protein on thermal stress in Lucilia cuprina作者机构：Center for Genomics Molecular & Human Genetics Jiwaji University Gwafior 474011 Cytogenetics Laboratory Department of Zoology Banaras Hindu University Varanasi 221005 India
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2016年第23卷第4期
页 面：555-568页
核心收录：
学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 071010[理学-生物化学与分子生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：Department of Science and Technology (DST) SERC program Govt. of India New Delhi
主 题：heat shock protein Hsp27 Lucilia cuprina thermal stress
摘 要：Hsp27, a highly conserved small molecular weight heat shock protein, is widely known to be developmentally regulated and heat inducible. Its role in thermotolerance is also implicated. This study is a sequel of our earlier studies to understand the molecular organization of heat shock genes/proteins and their role in development and thermal adaptation in a sheep pest, Lucilia cuprina （blowfly）, which exhibits unusually high adaptability to a variety of environmental stresses, including heat and chemicals. In this report our aim was to understand the evolutionary relationship of Lucilia hsp27 gene/protein with those of other species and its role in thermal adaptation. We sequence characterized the Lchsp27 gene （coding region） and analyzed its expression in various larval and adult tissues under normal as well as heat shock conditions. The nucleotide sequence analysis of 678 bps long-coding region of Lchsp27 exhibited closest evolutionary proximity with Drosophila （90.09%）, which belongs to the same order, Diptera. Heat shock caused significant en- hancement in the expression ofLchsp27 gene in all the larval and adult tissues examined, however, in a tissue specific manner. Significantly, in Malpighian tubules, while the heatinduced level ofhsp27 transcript （mRNA） appeared increased as compared to control, the protein level remained unaltered and nuclear localized. We infer that Lchsp27 may have significant role in the maintenance of cellular homeostasis, particularly, during summer months, when the fly remains exposed to high heat in its natural habitat.
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