Use of electroporation as an option to transform the horn fly, Haematobia irritans： a species recalcitrant to microinjection
Use of electroporation as an option to transform the horn fly, Haematobia irritans： a species recalcitrant to microinjection作者机构：Department of Biology Abilene Christian University Abilene TX 79699 USA USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory 2700 Fredericksburg Rd. Kerrville TX 78028 USA USDA-ARS Veterinary Pest Genomics Center Kerrville TX 78028 USA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2016年第23卷第4期
页 面：621-629页
核心收录：
学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 071010[理学-生物化学与分子生物学] 07[理学] 09[农学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：U.S. Department of Agriculture-APHIS [58-6205-0-038] Abilene Christian University Math and Science Grants
主 题：DsRed EGFP electroporation Haematobia irritans horn fly transformation transgenic insect
摘 要：The horn fly, Haematobia irritans, is a serious pest of cattle in North America. The control of horn flies has primarily relied on insecticides. However, the heavy use of insecticides has led to the development of insecticide resistance in horn flies. Novel methods to control horn flies are greatly needed. Transgenic technology is an effective tool to genetically modify insects and may lead to novel methods of pest control based on genomic approaches. Here we report apiggyBac-mediated transformation of the horn fly via electroporation. Transformation with a DsRed fluorescent marker protein coding region was verified by PCR analysis of individual fly bodies and pupal cases and sequencing of PCR products. However, Southern blot analysis failed to indicate the DsRed gene was integrated into the horn fly genome. Thus, the electroporation protocol may have caused the DsRed gene to be integrated into bacterial symbionts of the horn fly.
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