Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation:A preliminary study

作     者：Md.Moklesur Rahman Sarker Long Chiau Ming Md.Zaidul Islam Sarker M Shahabuddin Kabir Choudhuri 

作者机构：Department of ImmunochemistryDivision of Pharmaceutical SciencesOkayama University Graduate School of MedicineDentistry and Pharmaceutical Sciences Department of PharmacologyFaculty of PharmacyLincoln University College Faculty of PharmacyBrain Degeneration and Therapeutics GroupPharmaceutical and Life Sciences Co Re Universiti Teknologi MARA Department of Pharmaceutical TechnologyFaculty of PharmacyInternational Islamic University Malaysia Department of PharmacyFaculty of Biological ScienceJahangirnagar University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2016年第6卷第7期

页      面：568-573页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：Partially Supported by Ministry of Higher Education and Universiti Teknologi MARA Malaysia(Grant No.LESTARI:600-RMI/DANA 5/3/LESTARI(52/2015) 

主　　题："Saribadi" "Anantamul Salsa" Ayurvedic medicine Immunostimulant Lymphocytes Splenocytes Immunoglobulin M antibody Proliferation Differentiation 

摘      要：Objective:To assess the immunopotentiality of Ayurvedic polyherbal preparations,Saribadi and Anantamul Salsa.Methods: Freshly prepared BALB/c mice splenocytes were cultured with Saribadi orAnantamul Salsa treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%,3.00% and 4.00%(v/v)] at 37C for 5 days. The immunoglobulin M(IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, *** contamination was assessed by treating the preparations with polymyxin ***: The doses of Saribadi [0.25%, 0.50%, 0.75% and 1.00%(v/v)] significantly increased IgM productions(0.966, 0.728, 0.695 and 0.615 mg/m L vs. control 0.265 mg/m L)and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density(OD) vs. control 0.162 OD]. Similarly, the doses of Anantamul Salsa [0.50%, 0.75%,1.00% and 1.50%(v/v)] promoted IgM productions(0.933, 0.919, 0.917 and 0.892 mg/m L vs. control 0.502 mg/m L) and the doses of Anantamul Salsa [0.50%, 0.75%, 1.00%,1.50%, 2.00%, and 3.00%(v/v)] stimulated lymphocytes proliferation(absorbance 0.395,0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of Saribadiand Anantamul Salsa was not retarded by the treatment of preparations with polymyxin ***: Immunomodulatory activity of Saribadi and Anantamul Salsa was unveiled for the first time. Saribadi and Anantamul Salsa possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM *** preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required.