Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae

作     者：Qing-Ji Shen Hakimi Kassim Yong Huang Hui Li Jing Zhang Guang Li Peng-Ye Wang Jun Yan Fangfu Ye Ji-Long Liu 

作者机构：Medical Research Council Functional Genomics UnitDepartment of PhysiologyAnatomy and GeneticsUniversity of Oxford Key Laboratory of Entomology and Pest Control EngineeringCollege of Plant ProtectionSouthwest University Key Laboratory of Soft Matter PhysicsBeijing National Laboratory for Condensed Matter PhysicsInstitute of PhysicsChinese Academy of Sciences CAS-MPG Partner Institute for Computational BiologyShanghai Institutes of Biological SciencesChinese Academy of Sciences 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2016年第43卷第6期

页      面：393-404页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：supported by the UK Medical Research Council (to J.L.L.)  China Scholarship Council-University of Oxford Scholarship (to Q.J.S)  Chinese Scholarship Council Studentship (to Y.H.)  Malaysia Government Scholarship (to H.K.)  the National Natural Science Foundation of China (No. 11304372) (to H.L.  F. Y and P.Y.W.) and anonymous donation (to J.L.L.) 

主　　题：CTP synthase Metabolic enzyme Cytoophidium Glycolysis Glutamine Intracellular compartmentation Saccharomyces cerevisiae 

摘      要：Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filamentforming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frameGFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases（Ura7p and Ura8p） and two asparagine synthetases（Asn1p and Asn2p） form filaments both in the cytoplasm and in the *** imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.