Hepatogenic differentiation of mesenchymal stem cells induced by insulin like growth factor-Ⅰ

作     者：Maryam Ayatollahi Masoud Soleimani Seyed Ziaadin Tabei Maryam Kabir Salmani 

作者机构：Transplant Research CenterNemazi HospitalShiraz University of Medical Sciences Hematology DepartmentFaculty of Medical SciencesTarbiat Modarres University Pathology DepartmentShiraz University of Medical Sciences Molecular Genetics DepartmentNational Institute of Genetic Engineering and Biotechnology 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2011年第3卷第12期

页      面：113-121页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：Supported by A grant from Stem Cell Organization:www.stem cell.ir 

主　　题：Mesenchymal stem cell Differentiation Hepatocyte Insulin-like growth factor 1 Human 

摘      要：AIM:To improve hepatic differentiation of human mesenchymal stem cell(MSC)using insulin growth factor 1(IGF-Ⅰ),which has important role in liver development,hepatocyte differentiation and ***:Bone marrow of healthy donors was aspirated from the iliac *** adherent cells expanded rapidly and were maintained with periodic passages until a relatively homogeneous population was *** identification of these cells was carried out by immunophenotype analysis and differentiation potential into osteocytes and *** effectively induce hepatic differentiation,we designed a protocol based on a combination of IGF-Ⅰ and liver specificfactors(hepatocyte growth factor,oncostatin M and dexamethasone).Morphological features,hepatic functions and cytological staining were assessed to evaluate transdifferentiation of human marrow-derived ***:Flow cytometric analysis and the differentiation potential into osteoblasts and adipocytes showed that more than 90% of human MSCs which were isolated and expanded were positive by specif ic markers and functional *** assessment and evaluation of glycogen storage,albumin and α-feto protein expression,as well as albumin and urea secretion revealed a statistically signif icant difference between the experimental groups and ***:In vitro differentiated MSCs using IGF-Ⅰwere able to display advanced liver metabolic functions,supporting the possibility of developing them as potential alternatives to primary hepatocytes.