Larvicidal activity,inhibition effect on development,histopathological alteration and morphological aberration induced by seaweed extracts in Aedes aegypti(Diptera:Culicidae)
Larvicidal activity,inhibition effect on development,histopathological alteration and morphological aberration induced by seaweed extracts in Aedes aegypti(Diptera:Culicidae)作者机构：Drug and Herbal Research CentreFaculty of PharmacyUniversiti Kebangsaan Malaysia School of BiosciencesTaylor's UniversityTaylor's Lakeside Campus Medical Entomology UnitInfectious Disease Research CentreInstitute for Medical Research
出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)
年 卷 期：2015年第8卷第12期
页 面：981-986页
核心收录：
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学]
主 题：Mosquito larvicidal activity Dengue vector Aedes a
摘 要：Objective:To investigate the larvicidal activity,inhibition effect on development,histopathological alteration and morphological aberration induced by the extracts derived from seaweeds Bryopsis pennuta(B.pennata),Sargassum binderi(S.binderi) and Padina australis in Aedes aegypti(Ae.aegypti) larvae and to characterize the phytochetnical components of the three seaweeds.Methods:Larvicidal activity of the seaweeds towards the larvae of Ae.aegypti was determined according to WHO.The inhibition effect of seaweeds was assessed by determining the mortality,adult emergence rate,larval and pupa duration of the treated larvae.Histopathological effect on midgut epithelium of larvae and morphological aberration induced by the methanol extracts were examined.Phytochemical analysis was done to determine the presence of alkaloids,saponins,steroids and terpenoids in the seaweeds.Results:Chloroform partition of B.pennata extract exhibited the strongest larvicidal activity(LC_(50) = 82.55 μg/mL).followed by methanol extract of B.pennata(LC_(50) = 160.07 μg/mL) and chloroform partition of S.binderi extract(LC_(50) = 192.43 μg/mL).The methanol extract of S.bituleri exhibited the strongest effect on prolongation of larval period(1.5-fold longer as compared to control) and resulted in strongest inhibition effect in adult emergence(98.67%).The histopathological study showed that larvae treated with seaweed extracts had cytopathological alteration of the midgut epithelium.The morphological observation revealed that the anal papillae and terminal spiracles of larvae were the common sites of aberrations.Conclusions:The study provided information on various effects of seaweed extracts on Ae.aegypti.Further investigation on identifying the active compounds and their mechanisms of action is recommended.
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