Functional interplay among the flavivirus NS3 protease, helicase, and cofactors

作     者：Kuohan Li Wint Wint Phoo Dahai Luo 

作者机构：Lee Kong Chian School of Medicine Nanyang Technological University 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2014年第29卷第2期

页      面：74-85页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 

主　　题：正链RNA病毒 蛋白酶 解旋酶 相互作用 因子 非结构蛋白 蛋白复合物 末端区域 

摘      要：Flaviviruses are positive-sense RNA viruses, and many are important human pathogens. Nonstructural protein 2B and 3 of the flaviviruses(NS2BNS3) form an endoplasmic reticulum(ER) membrane-associated hetero-dimeric complex through the NS2B transmembrane region. The NS2BNS3 complex is multifunctional. The N-terminal region of NS3, and its cofactor NS2B fold into a protease that is responsible for viral polyprotein processing, and the C-terminal domain of NS3 possesses NTPase/RNA helicase activities and is involved in viral RNA replication and virus particle formation. In addition, NS2BNS3 complex has also been shown to modulate viral pathogenesis and the host immune response. Because of the essential functions that the NS2BNS3 complex plays in the flavivirus life cycle, it is an attractive target for antiviral development. This review focuses on the recent biochemical and structural advances of NS2BNS3 and provides a brief update on the current status of drug development targeting this viral protein complex.