Expression and function of a new angiogenic factor AA98 target molecule at the maternal-embryonic boundary of rhesus monkey

作     者：WEIPeng ZHANGZhihong CHENXinlei YANXiyun LIUYixun 

作者机构：StateKeyLaboratoryofReproductiveBiologyInstituteofZoologyChineseAcademyofSciencesBeijing100080China InstituteofBiophysicsChineseAcademyofSciencesBeijing100080China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2003年第48卷第9期

页      面：881-886页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：This work was supported by the WHO/Rockefeller Fundation(Grant No.RF96020#78) the Knowledge Innovation Program of CAS(Grant No.KSCX-2-SW-201) the National“973”Program(Grant No.G1999055901) the National Natural Science Foundation of China(Grant No.30270196) 

主　　题：AA98 胎盘形成 入侵 恒河猴 基因表达 目标分子 动物实验 

摘      要：The target molecule of monoclonal antibody AA98 (AA for short) is a new vascular endothelial cell related factor and plays a role in angiogenesis as indicated by the previous data. To investigate its role in angiogenesis and placentation in primate, we examined its expression in the implantation sites on D17, 19, 28 and 34 of gestation in rhesus monkey by immunohistochemistry and Western immunoblot. Western blot analysis showed that the primary antibody used in this study was specific for its epitope. AA protein was mainly expressed in small blood vessels and in some cytotrophoblast cells. The AA staining was found mainly in the endothelial cells and vascular small *** observation supported the AA s role in angiogenesis. AA was spatio-temporarily expressed in cytotrophoblasts: weak in proliferating trophoblast within cell column and endovascular trophoblast, strong in trophoblastic subpopulation within the basal plate and vascular trophoblast; AA staining within the basal plate was down-regulated during early placentation. The shift of AA98 expression in extravillous trophoblasts suggestes a role of this new factor during the course of cytotrophoblast metastasis and spiral artery remodeling. The spatio-temporarily expression indicats that AA98 could be also used as a trophoblast cellular marker to characterize the acquisition of a vascular endothelial and invasive phenotype.