Molecular characterization,biological forms and sporozoite rate of Anopheles stephensi in southern Iran

作     者：Ali Reza Chavshin Mohammad Ali Oshaghi Hasan Vatandoost Ahmad Ali Hanafi-Bojd Ahmad Raeisi Fatemeh Nikpoor 

作者机构：Social Determinants of Health Research CenterUrmia University of Medical Sciences Department of Medical Entomology and Vector ControlSchool of Public HealthUrmia University of Medical Sciences(UMSU) Department of Medical Entomology and Vector ControlSchool of Public HealthTehran University of Medical Sciences(TUMS) 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第1期

页      面：47-51页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：financially supported by Tehran University of Medical Sciences(TUMS)(Grant No.89-01-27-10448) 

主　　题：Anopheles stephensi Mysorensis Type Intermediate mtDNA markers Molecular systematic Iran 

摘      要：Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(***)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in ***:Wild live *** samples were collected from different malarious areas in southern *** biological forms were identified based on number of *** characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II(mtDNA-COI/COII).The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR ***:Results showed that all three biological forms including mysorensis,intermediate and type are present in the study *** investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito ***:Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the *** of malaria parasite infection in An,stephensi,the most important vector of malaria,may be partly due to the success and achievement of ongoing active malaria control program in the region.