蛋白A连续床亲和毛细管色谱柱的制备及性能考察

作     者：潘再法 莫卫民 吴仁安 罗权舟 叶明亮 邹汉法 

作者机构：浙江工业大学化工学院杭州310014 中国科学院大连化学物理研究所国家色谱研究分析中心大连116011 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：2002年第23卷第11期

页      面：2070-2072页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：国家杰出青年基金 (批准号 :2 972 5 5 12 )资助 

主　　题：制备 性能 亲和色谱法 连续床毛细管色谱柱 蛋白A 人免疫球蛋白G 分析 

摘      要：An assay technique for the determination of the human IgG in human plasma has been developed by utilizing protein A immobilized monolithic capillary column. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. Reactive monolithic capillary columns have been prepared by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the monolithic rods directly or through a 11 carbon atom space arm with molded synthetic methods. The non-specific adsorption of two kinds of media has been studied and the results showed that the medium without space arm gives very low non-specific adsorption of BSA. The affinity column without space arm was used to determine the human immunoglobulin G(HIgG) in human serum. The correlative coefficient of the calibration curve reaches 0 998 7. The total time of rapid analysis was less than 0 7 min. The consumption of eluent buffer and sample is much low than by conventional HPLC.