对来自Barrett食管的细胞学标本进行荧光原位杂交:初步可行性研究

作     者：Falk G.W. Skacel M. Gramlich T.L. 赵丽娜 

作者机构：Ctr. Swallowing Esopha geal Disord. Depts. Gastroenterol.Hepatol. C. Cleveland Clinic Foundation Cl eveland OHUnited StatesDr. 

出 版 物：《世界核心医学期刊文摘（胃肠病学分册）》 (Core Journals in Gastroenterology)

年 卷 期：2005年第1卷第2期

页      面：32-33页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Barrett 荧光原位杂交 初步可行性研究 不典型增生 阴性样 阴性患者 刷检 非整倍体 生物标记 

摘      要：Background Endoscopic brush cytology is a promising surveillance technique for Barrett’s esophagus. However, there is a need for ancillary biomarkers to incr ease the sensitivity of cytology and to allow identification of patients at increased risk for disease progression. The aims of this study were to eval uate the feasibility of fluorescence in situ hybridization of endoscopic brush c ytology specimens and to determine if there are specific chromosomal changes in cytologic specimens from patients with cancer that are not present in patients w ithout dysplasia. Methods Archival cytology slides from 16 patients with Barrett ’s esophagus were studied: 8 negative for dysplasia and 8 positive for adenocar cinoma. Fluorescence in situ hybridization was used to detect two alterations: H ER-2 gene(17q11.2-q12) and 20q13.2 region amplification. Observations For 7 of 8 adenocarcinoma cases, there was amplification/aneusomy of at least one of the two analyzed regions by fluorescence in situ hybridization. None of the samples negative for dysplasia were abnormal for either of the two genomic regions stud *** Fluorescence in situ hybridization is feasible by using routine Barrett’s esophagus cytologic specimens. Differences in genomic makeup can be d etected in cells from patients negative for dysplasia and in those with adenocar cinoma.