Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells
Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells作者机构:Department of Gastroenterology Xijing Hospital Fourth Military Medical University Xi'an 710033 Shaanxi Province China.
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2003年第9卷第5期
页 面:894-898页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:National Natural Science Foundation of China No. 30030140
主 题:ZNRD1基因 胃癌 耐药 反义RNA 癌细胞 聚合酶链反应 RT-PCR
摘 要:AIM: To investigate the expression level of ZNRD1 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR, and to observe the drug sensitizing and proliferation effect of ZNRD1 antisense nucleic acid transduction on SGC7901/VCR ***: Amplification of sequences encoding ZNRD1 from SGC7901/VCR cDNA by PCR. The levels of ZNRD1 mRNA expression were demonstrated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR).Eukaryotic expression vector pcDNA3.1-anti ZNRD1 was constructed and transfected into SGC7901/VCR cells by lipofectamine. Immunochemical method was used to detect the expression of protein in SGC7901/VCR cells and transfectants. The cell cycle alteration and the intracellular adriamycin (ADM) accumulation were observed by *** curve and drug sensitization of cells for vincristine (VCR) were analyzed with MTT ***: We cloned the open reading frame of full-length ZNRD1. The expression of ZNRD1 showed higher in SGC7901/VCR than in SGC7901 cells. The antisense ZNRD1 drug-resistant clones were selected after gene *** results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than that in non-transfectants. Comparing to SGC7901/VCR and pcDNA3.1-SGC7901/VCR, anti ZNRD1-SGC7901/VCR showed gradually accumulated in G1 phase, with aconcomitant decrease of cell population in S phase. FACSalso suggested intracellular ADM accumulation increased2fold in SGC7901/VCR cells after transfected with *** assay showed that transfectant cells proliferationwas lagged and more sensitive to vcr than ***: ZNRD1 gene displayed highly expression in VCR resistant gastric cancer *** of ZNRD1 protein was effectively blockde in anti ZzNRD1-SGC7901/VCR cells by gene ***1 antisense nucleic acid transfection sensitized drug resistant gastric cancer cells to VCR,increased AD