A simple and purification-free nucleic acid extraction method for rapid diagnosis of malaria
作者机构:Department of ParasitologyFaculty of MedicineUniversiti Malaya50603 Kuala LumpurMalaysia Vector Borne Disease SectorMinistry of HealthPutrajayaMalaysia Lipis District Health OfficePahangMalaysia State Health DepartmentNegeri SembilanMalaysia Microbiology SectorHospital Kuala Kubu BharuSelangorMalaysia
出 版 物:《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志(英文版))
年 卷 期:2023年第16卷第9期
页 面:428-430页
核心收录:
学科分类:1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金:supported by Long Term Research Grant Scheme (LRGS) LRGS/1/2018/UM/01/1/4 from the Ministry of Higher Education Malaysia
摘 要:In a point of care setting for the diagnosis of malaria,DNA extraction using conventional methods are time-consuming and ***,in this study we aim to utilize a simple nucleic acid extraction method to directly extract DNA from *** would in turn reduce the time,cost and equipment needed to perform DNA *** method is then coupled with LAMP assay for rapid diagnosis of malaria.