HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus
作者机构：State Key Laboratory of Plant Cell and Chromosome EngineeringInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic InteractionsCollege of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China Department of Nutritional Sciences&ToxicologyUniversity of CaliforniaBerkeleyCA 94720USA Lushan Botanical GardenChinese Academy of ScienceJiujiangJiangxi 332900China
出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)
年 卷 期：2024年第51卷第3期
页 面：313-325页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学]
基 金：funded by the National Key R&D Program of China(2018YFD1000703 and 2018YFD1000700) the Strategic Priority Research Program of Chinese Academy of Sciences(XDPB16) National Program on Research and Development of Transgenic Plants(2016ZX08009-003-001)
主 题：Barley MPK4 WRKY1 Phosphorylation Disease resistance Powdery mildew
摘 要：Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant ***,the functions of MAPK signaling pathways in crop disease resistance are largely *** we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune ***4 is identified to play a negative role in barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh ***,the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1^(DD) variant specifically phosphorylates HvMPK4 in ***,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1^(DD).Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by ***1 is phosphorylated in barley at the early stages of Bgh infection,which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression *** data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew.
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