检索结果-南通市图书馆

Generating homozygous mutant populations of Barley microspores by ethyl methanesulfonate treatment

aBIOTECH 2023年第3期4卷 202-212页

作者： Linli Huang Guangqi Gao Congcong Jiang Guimei Guo Qiang He Yingjie Zong Chenghong Liu Ping Yang Biotech Research Institute Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai 201106China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Induced mutations are important for genetic research and breeding.Mutations induced by physical or chemical mutagenesis are usually heterozygous during the early generations.However,mutations must be fixed prior to phenotyping or field trials,which requires additional rounds of self-pollination.Microspore culture is an effective method to produce double-haploid(DH)plants that are fixed homozygotes.In this study,we conducted ethyl methanesulfonate(EMS)-induced mutagenesis of microspore cultures of Barley(Hordeum vulgare)cultivar‘Hua30’and landrace‘HTX’.The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant regeneration.The two genotypes showed different regeneration efficiencies.The phenotypic variation of the regenerated M1 plants and the presence of genome-wide nucleotide mutations,revealed by whole-genome sequencing,highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH mutants.Genome-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture(somaclonal variation)rather than EMS-induced mutagenesis.In addition to producing a population of 1972 homozygous mutant lines that are available for future field trials,this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations(mainly by fine-tuning the mutagen dosage).

关键词： Barley Mutagenesis Microspore culture Double-haploid(DH) Homozygous mutant

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of Barley immunity to powdery mildew fungus

引用

Journal of Genetics and Genomics 2024年第3期51卷 313-325页

作者： Pengya Xue Ling Zhang Renchun Fan Yanan Li Xinyun Han Ting Qi Lifang Zhao Deshui Yu Qian-Hua Shen State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China Department of Nutritional Sciences&Toxicology University of CaliforniaBerkeleyCA 94720USA Lushan Botanical Garden Chinese Academy of ScienceJiujiangJiangxi 332900China

Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant species.However,the functions of MAPK signaling pathways in crop disease resistance are largely unknown.Here we report the function of HvMKK1-HvMPK4-HvWRKY1 module in Barley immune system.HvMPK4 is identified to play a negative role in Barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh infection.Furthermore,the Barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1^(DD) variant specifically phosphorylates HvMPK4 in vitro.Moreover,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1^(DD).Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by HvMPK4.HvWRKY1 is phosphorylated in Barley at the early stages of Bgh infection,which enhances its suppression on Barley immunity likely due to enhanced DNA-binding and transcriptional repression activity.Our data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate Barley immunity against powdery mildew.

关键词： Barley MPK4 WRKY1 Phosphorylation Disease resistance Powdery mildew

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exome-wide variation in a diverse Barley panel reveals genetic associations with ten agronomic traits in Eastern landraces

引用

Journal of Genetics and Genomics 2023年第4期50卷 241-252页

作者： June-Sik Kim Kotaro Takahagi Komaki Inoue Minami Shimizu Yukiko Uehara-Yamaguchi Asaka Kanatani Daisuke Saisho Ryuei Nishii Alexander ELipka Takashi Hirayama Kazuhiro Sato Keiichi Mochida Bioproductivity Informatics Research Team RIKEN Center for Sustainable Resource ScienceYokohama 230-0045Japan Institute of Plant Science and Resources Okayama UniversityKurashiki 710-0046Japan Kihara Institute for Biological Research Yokohama City UniversityYokohama 244-0813Japan Graduate School of Nanobioscience Yokohama City UniversityYokohama 236-0027Japan School of Information and Data Sciences Nagasaki UniversityNagasaki 852-8521Japan Department of Crop Sciences University of IllinoisUrbanaIL 61801USA Microalgae Production Control Technology Laboratory RIKEN Baton Zone ProgramRIKEN Cluster for ScienceTechnology and Innovation HubYokohama 230-0045Japan

Barley(Hordeum vulgare ssp.vulgare)is one of the first crops to be domesticated and is adapted to a wide range of environments.Worldwide Barley germplasm collections possess valuable allelic variations that could further improve Barley productivity.Although Barley genomics has offered a global picture of allelic variation among varieties and its association with various agronomic traits,polymorphisms from East Asian varieties remain scarce.In this study,we analyze exome polymorphisms in a panel of 274 Barley varieties collected worldwide,including 137 varieties from East Asian countries and Ethiopia.We reveal the underlying population structure and conduct genome-wide association studies for 10 agronomic traits.Moreover,we examin genome-wide associations for traits related to grain size such as awn length and glume length.Our results demonstrate the value of diverse Barley germplasm panels containing Eastern varieties,highlighting their distinct genomic signatures relative to Western subpopulations.

关键词： Barley Exome-seq Genome-wide association study(GWAS) Population structure Allelic variation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of Barley inclusion method and protease supplementation on growth performance,nutrient utilisation,and gastrointestinal tract development in broiler starters

引用

Animal Nutrition 2022年第1期8卷 61-70页

作者： L.M.Tari W.N.U.Perera F.Zaefarian M.R.Abdollahi A.J.Cowieson V.Ravindran Monogastric Research Centre School of Agriculture and EnvironmentMassey UniversityPrivate Bag 11222Palmerston North4442New Zealand Department of Animal Science Faculty of AgricultureUniversity of PeradeniyaPeradeniya20400Sri Lanka DSM Nutritional Products Wurmisweg 576KaiseraugstSwitzerland

The influence of the method of Barley inclusion(fine,coarse and whole Barley)in a wheat-based diet and protease supplementation(0 and 0.20 g/kg)on growth performance,nutrient utilisation and gastrointestinal tract development of broilers(d 1 to 21)was evaluated in a 32 factorial arrangement.Whole Barley(WB)grains were ground in a hammer mill to pass through the screen sizes of 2.5 and 8.0 mm to achieve fine(FB)and coarse(CB)Barley particle sizes,respectively.A total of 288,one-day-old male broilers were allotted to 36 cages(6 cages/treatment;8 birds/cage).There was no significant(P>0.05)interaction between Barley inclusion method and protease for any growth performance or nutrient utilisation parameters.Birds fed diets containing CB and WB showed higher(P0.05)on growth performance and nutrient utilisation,most likely due to the well balanced digestible amino acids and high inherent digestibility of protein in the basal diet,and/or the presence of exogenous carbohydrase and phytase.In conclusion,the present results showed that the inclusion of coarsely ground and whole Barley in a wheat-based diet can enhance nutrient and energy utilisation and is beneficial to the growth performance of young broilers.

关键词： Barley Broiler Particle size Protease Whole grain

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in Barley

引用

Journal of Integrative Plant Biology 2022年第3期64卷 756-770页

作者： Sakura D.Karunarathne Yong Han Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityPerthWA6150 Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityPerthWA6150 Australia Department of Primary Industries and Regional Development PerthWA6151 Australia

Nitrogen is a major determinant of grain yield and quality.As excessive use of nitrogen fertilizer leads to environmental pollution and high production costs,improving nitrogen use efficiency(NUE)is fundamental for a sustainable agriculture.Here,we dissected the role of the Barley abnormal cytokinin response1 repressor 1(Hv ARE1)gene,a candidate for involvement in NUE previously identified in a genome-wide association study,through natural variation analysis and clustered regularly interspacedshort palindromic repeats(CRISPR)/CRISPRassociated protein 9(Cas9)-mediated gene editing.Hv ARE1 was predominantly expressed in leaves and shoots,with very low expression in roots under low nitrogen conditions.Agrobacterium-mediated genetic transformation of immature embryos(cv.Golden Promise)with single guide RNAs targeting Hv ARE1 generated 22 T0 plants,from which four T1 lines harbored missense and/or frameshift mutations based on genotyping.Mutant are1 lines exhibited an increase in plant height,tiller number,grain protein content,and yield.Moreover,we observed a 1.5-to2.8-fold increase in total chlorophyll content in the flag leaf at the grain filling stage.Delayed senescence by 10–14 d was also observed in mutant lines.Barley are1 mutants had high nitrogen content in shoots under low nitrogen conditions.These findings demonstrate the potential of ARE1 in NUE improvement in Barley.

关键词： ARE1 Barley CRISPR/Cas9 gene editing leaf senescence NUE

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characterization of Rsg2.a3:A new greenbug resistance allele at the Rsg2 locus from wild Barley(Hordeum vulgare ssp.spontaneum)

引用

The Crop Journal 2022年第6期10卷 1727-1732页

作者： Xiangyang Xu Dolores Mornhinweg Amy Bernardo Genqiao Li Ruolin Bian Brian J.Steffenson Guihua Bai USDA-ARS Wheat Peanutand Other Field Crop Research UnitStillwaterOK 74075USA USDA-ARS Hard Winter Wheat Genetics Research Unit ManhattanKS 66506USA Department of Plant Pathology Kansas State UniversityManhattanKS 66506USA Department of Plant Pathology University of MinnesotaSt.PaulMN 55108USA

Greenbug(Schizaphis graminum Rondani)is a destructive insect pest that not only damages plants,but also serves as a vector for many viruses.Host plant resistance is the preferred strategy for managing greenbug.Two greenbug resistance genes,Rsg1 and Rsg2,have been reported in Barley.To breed cultivars with effective resistance against various greenbug biotypes,additional resistance genes are urgently needed to sustain Barley production.Wild Barley accession WBDC053(PI 681777)was previously found to be resistant to several greenbug biotypes.In this study,a recombinant inbred line(RIL)population derived from Weskan×WBDC053 was evaluated for response to two greenbug biotypes(E and TX1)and genotyped using genotyping by sequencing(GBS).A set of 3347 high quality GBS-derived single nucleotide polymorphisms(SNPs)were then used to map the greenbug resistance gene in this wild Barley accession.Linkage analysis placed the greenbug resistance gene in a 2.35 Mb interval(0-2,354,645 bp)in the terminal region of the short arm of chromosome 2H.This interval harbors 15 genes with leucine-rich-repeat(LRR)protein domains.An allelism test indicated that the greenbug resistance gene in WBDC053,designated Rsg2.a3,is likely allelic or closely linked to Rsg2.GBS-SNPs 2H_1318811and 2H_1839499 co-segregating with Rsg2.a3 in the RIL population were converted to Kompetitive allele specific PCR(KASP)markers KASP-Rsg2.a3-1 and KASP-Rsg2.a3-2,respectively.The two KASP markers can be used to select Rsg2.a3 and have the potential to tag Rsg2 in Barley improvement programs.

关键词： Barley Greenbug resistance gene KASP markers Genotyping by sequencing Linkage mapping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in studies on the physiological and molecular regulation of Barley tillering

引用

Journal of Integrative Agriculture 2023年第1期22卷 1-13页

作者： Asad RIAZ Ahmad M.ALQUDAH Farah KANWAL Klaus PILLEN YE Ling-zhen DAI Fei ZHANG Guo-ping Department of Agronomy College of Agriculture and BiotechnologyZhejiang UniversityZijingang CampusHangzhou 310029P.R.China Department of Agroecology Aarhus University at Flakkebjerg4200 SlagelseDenmark Institute of Agricultural and Nutritional Sciences Martin-Luther-University Halle-WittenbergBetty-Heimann-Straße 306120 Halle(Saale)Germany

Tillering is a crucial trait closely associated with yield potential and environmental adaptation in cereal crops,regulated by the synergy of endogenous(genetic)and exogenous(environmental)factors.The physiological and molecular regulation of tillering has been intensively studied in rice and wheat.However,tillering research on Barley is scarce.This review used the recent advances in bioinformatics to map all known and potential Barley tiller development genes with their chromosomal genetic and physical positions.Many of them were mapped for the first time.We also discussed tillering regulation at genetic,physiological,and environmental levels.Moreover,we established a novel link between the genetic control of phytohormones and sugars with tillering.We provided evidence of how environmental cues and cropping systems help optimize the tiller number.This comprehensive review enhances the understanding of Barley’s physiological and genetic mechanisms controlling tillering and other developmental traits.

关键词： Barley development genetic regulation phytohormone tillering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fine-mapping and characterisation of genes on Barley(Hordeum vulgare)chromosome 2H for salinity stress tolerance during germination

引用

The Crop Journal 2022年第3期10卷 754-766页

作者： Edward Mwando Yong Han Tefera Angessa Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityMurdochWA 6150Australia Department of Primary Industries and Regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia

Salinity causes a detrimental impact on plant growth,particularly when the stress occurs during germination and early development stages.Barley is one of the most salt-tolerant crops;previously we mapped two quantitative trait loci(QTL)for salinity tolerance during germination on the short arm of chromosome 2 H using a CM72/Gairdner doubled haploid(DH)population.Here,we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to Barley reference genomes v1 and v3 respectively,using two DH populations of CM72/Gairdner and Skiff/CM72,F_(2)and F;generations of CM72/Gairdner/;Spartacus CL,Two Receptorlike kinase 4(RLPK4)v1 or Receptor-like kinase(RLK)v3 could be the candidates for enhanced germination under salinity stress because of their upregulated expression in salt-tolerant variety CM72.Besides,several insertion/deletion polymorphisms were identified within the 3 rd exon of the genes between CM72 and Gairdner.The sequence variations resulted in shifted functional protein domains,which may be associated with differences in salinity tolerance.Two molecular markers were designed for selecting the locus with receptor-like protein kinase 4,and one was inside HORVU2 Hr1 G111760.1 or HORVU.MOREX.r3.2 HG0202810.1.The diagnostic markers will allow for pyramiding of 2 H locus in Barley varieties and facilitate genetic improvement for saline soils.Further,validation of the genes to elucidate the mechanisms involved in enhancing salinity tolerance at germination and designing RLPK4 specific markers is proposed.For this publication,all the analysis was based on Barley reference genome of2017(v1),and it was used throughout for consistence.However,the positions of the markers and genes identified were updated according to new genome(v3)for reference.

关键词： Barley Germination Salinity tolerance Diagnostic markers Receptor-like protein kinase 4 Gene expression Sequence analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic analysis and gene mapping of a dwarf and liguleless mutation in Barley

引用

The Crop Journal 2022年第4期10卷 1094-1102页

作者： Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009JiangsuChina Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou 225009JiangsuChina Key Laboratory of Crop Germplasm Resources and Utilization(MOA) The National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement.However,most genes controlling leaf development in Barley remain unknown.We identified a dwarf and liguleless(dl)mutant derived by ethylmethane sulfonate mutagenesis.The dl mutant showed dramatic changes in shoot architecture compared with wild-type(Yangnongpi 5)plants.Besides lacking ligules,the dl mutant showed much shorter plant height(28 cm)than Yangnongpi 5(78 cm).By map-based cloning,the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7.A C-to-T single-nucleotide substitution was identified at exon position 790,and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960.Consequently,HORVU7Hr1G106960 was identified as the DL gene,encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1(ALOG)domain.DL is highly similar to rice OsG1-LIKE 1/2(OsG1L1/2)and sorghum AWN1/AWN1-10 at the amino acid level.Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR,we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in Barley.

关键词： Barley EMS Liguleless ALOG Shoot architecture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Rate and Source Organo-Mineral Material on Forage Yield and Nutritive Value of Barley-Pea Mixed under Arid Conditions

引用

Phyton-International Journal of Experimental Botany 2022年第7期91卷 1483-1493页

作者： Walid Soufan Nasser A.Al-Suhaibani Plant Production Department College of Food and Agriculture SciencesKing Saud UniversityRiyadh11451Saudi Arabia

Cereal-legume intercropping plays a vital role in the subsistence food production system that prevails in the arid regions.It not only provides profitable crop productivity for agricultural communities but also plays an important role in improving soil fertility.Therefore,the present research was conducted to assess the effect of the organic and mineral fertilizers on the forage yield and nutritional value in Barley-pea intercropping system.The results revealed that the quality of forage grass is significantly influenced by both organic and inorganic fertilizer.Thus,organic fertilizer application has significantly influenced the dry matter(DM),crude ash(CA),crude protein(CP),neutral detergent fiber(NDF),and acid detergent fiber(ADF).Among the treatments,organic fertilizer added at rate of 35 m^(−3) ha^(−1) produced the maximum fresh and dry matter in Barley and peas.In addition,the same level of organic fertilizer also improved silage composition by significantly increasing the protein and fiber content and showed highest the values.Based on overall results,it is concluded that organic fertilizer treatment(35 m^(−3) ha^(−1))has the potential as an effective strategy to improve the productivity and nutritional quality of the Barley-pea intercropping system in arid areas.The results revealed that organic fertilizer can be utilized in sustainable agricultural as a source of nutrients for numerous various crops under arid conditions.

关键词： Organic fertilizer Barley pea yield quality hay silage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：