Development and mapping of SSR markers linked to resistance-gene homologue clusters in common bean

作     者：Luz Nayibe Garzon Matthew Wohlgemuth Blair 

作者机构：Universidad Nacional de Colombia Dept. de Agronomia Av. El Dorado Bogotá and Dept. de Ciencias Agricolas Km. 12 Via ChapineroPalmira Colombia Universidad de Cundinamarca Facultad de Ciencias agropecuarias Programa Ingeniería Agronómica Facatativá-Cundinamarca Universidad Nacional de Colombia Dept. de Ciencias Agricolas Km. 12 Chapinero Palmira Colombia Tennessee State University Department of Agriculture and Natural Science Lawson Hall 3500 John A. Merritt Blvd. NashvilleTN 37219 USA 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2014年第2卷第4期

页      面：183-194页

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：CENICAFE Pilar Moncada Univ. of California–Davis 

主　　题：Bacterial artificial chromosome(BAC) clone end sequences(BES) Simple sequence repeats(SSRs) Plant disease resistance(R) genes Nucleotide binding site targeted sequencing Resistance gene analogs 

摘      要：Common bean is an important but often a disease-susceptible legume crop of temperate,subtropical and tropical regions worldwide. The crop is affected by bacterial, fungal and viral pathogens. The strategy of resistance-gene homologue(RGH) cloning has proven to be an efficient tool for identifying markers and R(resistance) genes associated with resistances to diseases. Microsatellite or SSR markers can be identified by physical association with RGH clones on large-insert DNA clones such as bacterial artificial chromosomes(BACs). Our objectives in this work were to identify RGH-SSR in a BAC library from the Andean genotype G19833 and to test and map any polymorphic markers to identify associations with known positions of disease resistance genes. We developed a set of specific probes designed for clades of common bean RGH genes and then identified positive BAC clones and developed microsatellites from BACs having SSR loci in their end sequences. A total of 629 new RGH-SSRs were identified and named BMr(bean microsatellite RGH-associated markers). A subset of these markers was screened for detecting polymorphism in the genetic mapping population DOR364 × G19833. A genetic map was constructed with a total of 264 markers,among which were 80 RGH loci anchored to single-copy RFLP and SSR markers. Clusters of RGH-SSRs were observed on most of the linkage groups of common bean and in positions associated with R-genes and QTL. The use of these new markers to select for disease resistance is discussed.