Construction and Expression of Sugarcane UGPase cDNA Prokaryotic Expression Vector
Construction and Expression of Sugarcane UGPase cDNA Prokaryotic Expression Vector作者机构：Key Laboratory of Hybrid Rice Germplasm Innovation and Molecular Breeding of South China Ministry of Agriculture P.R.China/FuzhouBranch National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian KeyLaboratory of Rice Molecular Breeding~Rice Research Institute Fujian Academy of Agricultural Sciences Fuzhou 350003 China College of Life Sciences Fujian Normal University Fuzhou 350108 China Key Laboratory of Sugarcane Genetics and Breeding Ministry of Agriculture Fuzhou 350108 China
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2011年第5卷第12期
页 面：981-985页
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
主 题：UGPase construction of prokaryotic expression vector induced expression.
摘 要：UGPase （UDP-glucose pyrophosphorylase）, one of the primary enzymes concerned with carbohydrate metabolism, catalyzes the formation of UDPG. By inserting the UGPase cDNA fragment cloned from Saccharum officinarum into PQE-30, the prokaryotic expression vector of PQE-UGP was successfully constructed. Then the vector plasmid of PQE-UGP was transformed into host bacteria M 15 and the expression of target gene was induced by Isopropyl β-D-1-Thiogalactopyranoside （IPTG）. The research laid foundation for study on the prokaryotic expression of UGPase.
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