RsERF40 contributes to cold stress tolerance and cell expansion of taproot in radish (Raphanus sativus L.)
作者机构：National Key Laboratory of Crop Genetics&Germplasm Enhancement and utilizationKey Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Horticulture and Landscape ArchitectureYangzhou UniversityYangzhou 225009China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第3期
页 面：223-234页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金：funded by grants from the National Key Technology R&D Program of China(2018YFD1000800) National Natural Science Foundation of China(32172579) Jiangsu Seed Industry Revitalization Project[JBGS(2021)071] the earmarked fund for Jiangsu Agricultural Industry Technology System[JATS(2022)463] Jiangsu Agricultural Science and Technology Innovation Fund(CX(21)2020) the Project Founded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
摘 要：The growth and development of taproots are inhibited by cold stress in radish(Raphanus sativus L.).Ethylene-responsive element binding factors(ERF)are key participators in the cold stress response and growth regulation of ***,the function of ERF genes in cold tolerance and root development in radish remains ***,we showed that the secondary growth of radish taproots was inhibited by cold *** transcriptome analysis demonstrated that the RsERF40 gene is an important regulator of the cold stress response and root growth *** cold tolerance of transgenic Arabidopsis plants overexpressing the RsERF40 gene was significantly *** RsERF40 in the cold-sensitive radish genotype and silencing RsERF40 in the cold-tolerant radish genotype indicated that RsERF40 was beneficial for alleviating oxidative damage under cold stress in *** Arabidopsis seedlings showed an increase in the elongation and radial growth of dark-grown ***-qPCR analysis showed that the expression of the cold-related genes(CORs)RsCOR78 and RsCOR413PM1 and the cell wall strengthening-related genes RsCESA6 and RsEXPB3 was upregulated in transgenic Arabidopsis *** one-hybrid(Y1H)and dual-luciferase reporter assays(DLA)revealed that RsERF40 directly regulates RsCOR78,RsCOR413PM1,RsCESA6 and RsEXPB3 expression,illustrating that RsERF40 enhances cold tolerance and taproot growth by modulating osmotic adjustment and cell wall mechanical strength in *** this study,the RsERF40-regulon was firstly found to be a new cold response pathway independent of the CBF-COR pathway conferring cold stress tolerance with increasing radish taproot *** results provided novel insight into the molecular mechanism underlying cold stress response and would facilitate the genetic improvement of cold tolerance in radish and other root vegetable crops.
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