Separation and purification of deoxynivalenol(DON) mycotoxin from wheat culture using a simple two-step silica gel column chromatography

作     者：ZHAO Xiu-mei LI Rong-jia ZHOU Chuang ZHANG Jie HE Cheng-hua ZHENG Ya-ting WU Wen-da ZHANG Hai-bin 

作者机构：College of Veterinary Medicine Nanjing Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第3期

页      面：694-701页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Natural Science Foundation of China (31402268) the Natural Science Foundation of Jiangsu Province of China (BK20140691) Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China The Introduction of International Advanced Agricultural Science and Technology Project from the Ministry of Agriculture of China (2012-Z22) 

主　　题：deoxynivalenol silica gel column chromatography separation purification 

摘      要：Deoxynivalenol（DON） is a type B trichothecenes mycotoxin produced by several Fusarium species, often found in foodstuffs for humans and animals. DON is in great demand for the toxicological researches both in vivo and in vitro. In this work, wheat culture was inoculated with a Fusarium graminearum PH-1 strain for DON production. The solvent system for crude extraction was acetonitrile-water（84：16, v/v）. A simple two-step silica gel column chromatography was employed to separate the DON mycotoxin from wheat culture, combined with preparative high performance liquid chromatography（preparative HPLC） to purify the compound. The solvent system for the second silica gel column chromatography was methylene chloride-methanol（17：1, v/v）, which provided a good elution effect selected on thin layer chromatography（TLC）. The target compound was identified by HPLC, and the chemical structure was confirmed by mass spectrometry（MS） and ~1H and ^（13）C nuclear magnetic resonance（NMR） spectroscopy. A total of 433 mg of purified DON was obtained from 1 kg of wheat culture, with a purity of 99.01%. The study had provided an easy-operating and cost-effective method to isolate an expensive compound in a simple way.