Analysis with NMR localized spectroscopy of rat brain in vivo and ex vivo

作     者：Guangliang Ding Xiaodong Zhang Liyun Li Chaohui Ye 

作者机构：Chinese Acad Sci Wuhan Inst Phys & Math Lab Magnet Resonance & Atom & Mol Phys Wuhan 430071 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1998年第43卷第2期

页      面：140-142页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：theNationalNaturalScienceFoundationofChina (GrantNo .195 740 6 7) 

主　　题：NMR localized spectroscopy rat brain. 

摘      要：The volume-selected MRS of rat brain was performed on a 200 MHz broad horizontal-bore nuclear magnetic resonance imaging spectrometer. The localized proton spectroscopy of rat brain was acquired using the double spin-echo pulse sequence (PRESS) to select the position and size of the volume. Water signal was suppressed by CHESS sequence which was composed of chemical shift selective rf pulses and dephasing gradients. The parameter TE was taken as 40 ms and 135 ms in the experiment. The localized MRS of the rat brain with the status of live and death was obtained in various TE values. Results showed that volume-selected proton MRS of the rat brain in vivo with longer TE consisted mainly of signals produced by N-acetylaspartate (NAA), creatine (Cre), choline (Cho) and the lactate spectral peak occurred in the MRS of the rat brain ex vivo. On the other hand, the MRS of the rat brain with shorter TE contained glutamine/glutamate and lipid signals besides those signals mentioned previously. Although the information abstracted from the MRS of the rat brain with shorter TE may be more than that with longer TE, the spectral resolution of the MRS with shorter TE was slightly worse. In addition, it might indicate that the absolute concentration of NAA was decreased in dead rat brain because the SNR of NAA peak in the MRS ex vivo got lower.