Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells

作     者：Fei Wang Shushu Zhu Xuefu Han Jindan Xu Jinnan Zhang Di Yang 

作者机构：Institute of Cardiovascular Disease the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2009年第23卷第4期

页      面：247-250页

学科分类：071010[理学-生物化学与分子生物学] 0808[工学-电气工程] 081704[工学-应用化学] 07[理学] 08[工学] 071009[理学-细胞生物学] 09[农学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0810[工学-信息与通信工程] 0817[工学-化学工程与技术] 0901[农学-作物学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by National Natural Science Founda-tion of China(30770894) Medical Foundation key scholar of Jiangsu Province(RC2007037) 

主　　题：endothelial cells C-reactive protein OX40L 

摘      要：Objective： To investigate the expression of OX40 ligand（OX40L） on C-reactive protein（CRP）-triggered mouse aorta endothelial cells （MAECs） in vitro. Methods： MAECs from aorta were isolated by digestion with collagenase type II. The cell growth was confirmed by morphological characteristics and the immunological marker, factor Ⅷ（or Willebrand factor, vWF）. The expression of OX40L by MAECs was detected by RT-PCR and western blot after incubating with 100μg/ml CRP for 48 hours. Results： Twenty-day cultures of MAECs formed confluent monolayer of a cobblestone pattern. RT-PCR and western blot assay showed that the level of OX40L expression in MAECs receiving CRP treatment was higher than control. Conclusion： A reliable method is described to isolate and propagate MAECs. CRP upregulates OX40L expression in MAECs.