Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates

作     者：Xiao-Li Feng Dandan Yu Mi Zhang Xiaohong Li Qing-Cui Zou Wentai Ma Jian-Bao Han Ling Xu Cuixian Yang Wang Qu Zhong-Hua Deng Junyi Long Yanghaopeng Long Mingkun Li Yong-Gang Yao Xing-Qi Dong Jianxiong Zeng Ming-Hua Li Xiao-Li Feng;Dandan Yu;Mi Zhang;Xiaohong Li;Qing-Cui Zou;Wentai Ma;Jian-Bao Han;Ling Xu;Cuixian Yang;Wang Qu;Zhong-Hua Deng;Junyi Long;Yanghaopeng Long;Mingkun Li;Yong-Gang Yao;Xing-Qi Dong;Jianxiong Zeng;Ming-Hua Li

作者机构：Kunming National High-level Biosafety Research Center for Non-Human PrimatesCenter for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunming650107China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciencesand KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyChinese Academy of SciencesKunming650201China National Resource Center for Non-Human PrimatesNational Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunming650107China Department of Infectious DiseasesYunnan Provincial Infectious Diseases HospitalKunming650301China Key Laboratory of Genomic and Precision MedicineBeijing Institute of GenomicsChinese Academy of Sciencesand China National Center for BioinformationBeijing100101China University of Chinese Academy of SciencesBeijing100049China Center for Excellence in Animal Evolution and GeneticsChinese Academy of SciencesKunming650201China Kunming College of Life ScienceUniversity of Chinese Academy of SciencesKunming650204China Yunnan Key Laboratory of Biodiversity InformationKunming Institute of ZoologyChinese Academy of SciencesKunming650201China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2022年第37卷第6期

页      面：804-812页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Major Science and Technique Programs in Yunnan Province(grant No.202102AA310055) the Bureau of Frontier Sciences and Education,CAS(grant no.QYZDJ-SSW-SMC005 to Y.G.Y.) the Key project of the CAS“Light of West China”Program(to D.Y.) Yunnan Province(202001AS070023 to D.Y.) Yunnan Fundamental Research Projects(grant No.202201AW070020 to J.Z.) 

主　　题：SARS-CoV-2 Variants of concern(VOC) Alpha Delta hACE2 transgenic mice Hamster 

摘      要：The continuously arising of SARS-CoV-2 variants has been posting a great threat to public health safety globally,from B.1.17(Alpha), B.1.351(Beta), P.1(Gamma), B.1.617.2(Delta) to B.1.1.529(Omicron). The emerging or reemerging of the SARS-CoV-2 variants of concern is calling for the constant monitoring of their epidemics,pathogenicity and immune escape. In this study, we aimed to characterize replication and pathogenicity of the Alpha and Delta variant strains isolated from patients infected in Laos. The amino acid mutations within the spike fragment of the isolates were determined via sequencing. The more efficient replication of the Alpha and Delta isolates was documented than the prototyped SARS-CoV-2 in Calu-3 and Caco-2 cells, while such features were not observed in Huh-7, Vero E6 and HPA-3 cells. We utilized both animal models of human ACE2(hACE2)transgenic mice and hamsters to evaluate the pathogenesis of the isolates. The Alpha and Delta can replicate well in multiple organs and cause moderate to severe lung pathology in these animals. In conclusion, the spike protein of the isolated Alpha and Delta variant strains was characterized, and the replication and pathogenicity of the strains in the cells and animal models were also evaluated.