Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces
作者机构：National Taiwan UniversityInstitute of Polymer Science and EngineeringTaipei 10617TaiwanChina National Health Research InstitutesInstitute of Cellular and System MedicineMiaoli 350TaiwanChina National Taiwan UniversityResearch and Development Center for Medical DevicesTaipei 10617TaiwanChina
出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)
年 卷 期：2022年第14卷第8期
页 面：616-632页
核心收录：
学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学]
基 金：National Taiwan University Core Consortium,No.NTU-CC-110L892501 Ministry of Science and Technology,No.MOST 110-2218-E-002-037
主 题：Cell membrane fluidity Cell-cell interaction Cellular spheroids Chitosan Hyaluronan
摘 要：BACKGROUND The therapeutic potential of mesenchymal stem cells(MSCs)in the form of threedimensional spheroids has been extensively *** underlying mechanisms for the altered cellular behavior of spheroids have also been *** membrane fluidity is a critically important physical property for the regulation of cell behavior,but it has not been studied for the spheroid-forming cells to *** To explore the association between cell membrane fluidity and the morphological changes of MSC spheroids on the surface of biomaterials to elucidate the role of membrane fluidity during the spheroid-forming process of *** We generated three-dimensional(3D)MSC spheroids on the surface of various culture substrates including chitosan(CS),CS-hyaluronan(CS-HA),and polyvinyl alcohol(PVA)*** cell membrane fluidity and cell morphological change were examined by a time-lapse recording system as well as a highresolution 3D cellular image *** and normal/cancer cells were prestained with fluorescent dyes and co-cultured on the biomaterials to investigate the exchange of cell membrane during the formation of heterogeneous cellular *** We discovered that vesicle-like bubbles randomly appeared on the outer layer of MSC spheroids cultured on different biomaterial *** average diameter of the vesicle-like bubbles of MSC spheroids on CS-HA at 37℃ was approximately 10μm,smaller than that on PVA substrates(approximately 27μm).Based on time-lapse images,these unique bubbles originated from the dynamic movement of the cell membrane during spheroid formation,which indicated an increment of membrane fluidity for MSCs cultured on these ***,the membrane interaction in two different types of cells with similar membrane fluidity may further induce a higher level of membrane translocation during the formation of heterogeneous *** Changes in cell membrane fluidity may be a novel path to elucidate the
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