A new rabbit model of implant-related biofilm infection： development and evaluation

作     者：Cheng-Bing CHU Hong ZENG Ding-Xia SHEN Hui WANG Ji-Fang WANG Fu-Zhai CUI 

作者机构：Beijing Chaoyang Hospital Capital Medical University Beijing 100043 China Department of Microbiology Chinese PLA General Hospital Beijing 100853 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Orthopedics Chinese PLA General Hospital Beijing 100853 China 

出 版 物：《Frontiers of Materials Science》 (材料学前沿（英文版）)

年 卷 期：2016年第10卷第1期

页      面：80-89页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Acknowledgements We thank the National Natural Science Foundation of China (Grant No. 21371106) and the Major State Basic Research Development Program of China (973 Program  2011CB606205) for the financial support. The authors would like to thank the Department of Microbiology of Chinese PLA General Hospital for bacterial culture experiments. Animal experiments were done at Laboratory Animal Center of Chinese PLA General Hospital. SEM and LCSM were performed at Tsinghna University 

主　　题：implant infection biofilm model rabbit 

摘      要：This study is to establish a rabbit model for human prosthetic joint infection and biofiim formation. Thirty-two healthy adult rabbits were randomly divided into four groups and implanted with stainless steel screws and ultra-high molecular weight polyethylene （UHMWPE） washers in the non-articular surface of the femoral lateral condyle of the right hind knees. The rabbit knee joints were inoculated with 1 mL saline containing 0, 102, 103, 104 CFU of Staphylococcus epidermidis （S. epidermidis） isolated from the patient with total knee arthroplasty （TKA） infection, respectively. On the 14th postoperative day, the UHMWPE washers from the optimal 103 CFU group were further examined. The SEM examination showed a typical biofilm construction that circular S. epidermidis were embedded in a mucous-like matrix. In addition, the LCSM examination showed that the biofilm consisted of the polysaccharide stained bright green fluorescence and S. epidermidis radiating red fluorescence. Thus, we successfully create a rabbit model for prosthetic joint infection and biofilm formation, which should be valuable for biofilm studies.